Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

Jin Woo Lee; Ji Hoon Lee; Chul Lee; Qinghao Jin; Dongho Lee; Youngsoo Kim; Jin Tae Hong; Mi Kyeong Lee; Bang Yeon Hwang

doi:10.1016/j.bmcl.2014.12.012

Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

Jin Woo Lee, Ji Hoon Lee, Chul Lee, Qinghao Jin, Dongho Lee, Youngsoo Kim, Jin Tae Hong, Mi Kyeong Lee, Bang Yeon Hwang

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC₅₀ values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

Original language	English
Pages (from-to)	960-962
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/j.bmcl.2014.12.012
Publication status	Published - 2015 Feb 15

Keywords

Leguminosae
Nitric oxide production inhibitor
Pterocarpan
Sophora tonkinensis

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2014.12.012

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title = "Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages",

abstract = "Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.",

keywords = "Leguminosae, Nitric oxide production inhibitor, Pterocarpan, Sophora tonkinensis",

author = "Lee, {Jin Woo} and Lee, {Ji Hoon} and Chul Lee and Qinghao Jin and Dongho Lee and Youngsoo Kim and Hong, {Jin Tae} and Lee, {Mi Kyeong} and Hwang, {Bang Yeon}",

note = "Funding Information: This research was supported financially by the Medical Research Center Program (MRC, 2008-0062275 ) through the National Research Foundation of Korea . Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2015",

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doi = "10.1016/j.bmcl.2014.12.012",

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AU - Lee, Jin Woo

AU - Lee, Ji Hoon

AU - Lee, Chul

AU - Jin, Qinghao

AU - Lee, Dongho

AU - Kim, Youngsoo

AU - Hong, Jin Tae

AU - Lee, Mi Kyeong

AU - Hwang, Bang Yeon

N1 - Funding Information: This research was supported financially by the Medical Research Center Program (MRC, 2008-0062275 ) through the National Research Foundation of Korea . Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2015/2/15

Y1 - 2015/2/15

N2 - Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

AB - Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4′,7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0 μM in RAW 264.7 macrophages.

KW - Leguminosae

KW - Nitric oxide production inhibitor

KW - Pterocarpan

KW - Sophora tonkinensis

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DO - 10.1016/j.bmcl.2014.12.012

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VL - 25

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 4

ER -

Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages

Abstract

Keywords

ASJC Scopus subject areas

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