Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose

Young Jun Shim; Ho Kyung Doo; Se Young Ahn; Yong Suk Kim; Je Kyung Seong; In Sun Park; Bon Hong Min

doi:10.1016/S0378-8741(02)00370-7

Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose

Young Jun Shim, Ho Kyung Doo, Se Young Ahn, Yong Suk Kim, Je Kyung Seong, In Sun Park, Bon Hong Min

Research output: Contribution to journal › Article › peer-review

223 Citations (Scopus)

Abstract

The present study examined the inhibitory effect of aqueous extract from the gall of Rhus chinensis (AEGRC) on alpha-glucosidase activity, an enzyme responsible for digestion of carbohydrate to monosaccharides in the process of intestinal absorption. AEGRC inhibited Bacillus alpha-glucosidase acitvity with an IC₅₀ of 0.9μg/ml. Its inhibition on alpha-glucosidase was determined to be noncompetitive and reversible when the enzyme-substrate mixture was simultaneously treated with AEGRC as an inhibitor. In addition, when it was orally administered to rats with sucrose (2g/kg), AEGRC (250-1000mg/kg) significantly suppressed the increase of blood glucose levels after sucrose loading in a dose dependent manner. These results suggest that AEGRC might exert anti-diabetic effect by suppressing carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.

Original language	English
Pages (from-to)	283-287
Number of pages	5
Journal	Journal of Ethnopharmacology
Volume	85
Issue number	2-3
DOIs	https://doi.org/10.1016/S0378-8741(02)00370-7
Publication status	Published - 2003 Apr

Keywords

Alpha-glucosidase inhibitor
Gall of Rhus chinensis
Postprandial hypoglycemic effect

ASJC Scopus subject areas

Pharmacology
Drug Discovery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0378-8741(02)00370-7

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title = "Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose",

abstract = "The present study examined the inhibitory effect of aqueous extract from the gall of Rhus chinensis (AEGRC) on alpha-glucosidase activity, an enzyme responsible for digestion of carbohydrate to monosaccharides in the process of intestinal absorption. AEGRC inhibited Bacillus alpha-glucosidase acitvity with an IC50 of 0.9μg/ml. Its inhibition on alpha-glucosidase was determined to be noncompetitive and reversible when the enzyme-substrate mixture was simultaneously treated with AEGRC as an inhibitor. In addition, when it was orally administered to rats with sucrose (2g/kg), AEGRC (250-1000mg/kg) significantly suppressed the increase of blood glucose levels after sucrose loading in a dose dependent manner. These results suggest that AEGRC might exert anti-diabetic effect by suppressing carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.",

keywords = "Alpha-glucosidase inhibitor, Gall of Rhus chinensis, Postprandial hypoglycemic effect",

author = "Shim, {Young Jun} and Doo, {Ho Kyung} and Ahn, {Se Young} and Kim, {Yong Suk} and Seong, {Je Kyung} and Park, {In Sun} and Min, {Bon Hong}",

note = "Funding Information: This study was supported by a grant of the Oriental Medicine R&D Project, Ministry of Health & Welfare, Republic of Korea (HMP-00-CO-06-0006).",

year = "2003",

month = apr,

doi = "10.1016/S0378-8741(02)00370-7",

language = "English",

volume = "85",

pages = "283--287",

journal = "Journal of Ethnopharmacology",

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TY - JOUR

T1 - Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose

AU - Shim, Young Jun

AU - Doo, Ho Kyung

AU - Ahn, Se Young

AU - Kim, Yong Suk

AU - Seong, Je Kyung

AU - Park, In Sun

AU - Min, Bon Hong

N1 - Funding Information: This study was supported by a grant of the Oriental Medicine R&D Project, Ministry of Health & Welfare, Republic of Korea (HMP-00-CO-06-0006).

PY - 2003/4

Y1 - 2003/4

N2 - The present study examined the inhibitory effect of aqueous extract from the gall of Rhus chinensis (AEGRC) on alpha-glucosidase activity, an enzyme responsible for digestion of carbohydrate to monosaccharides in the process of intestinal absorption. AEGRC inhibited Bacillus alpha-glucosidase acitvity with an IC50 of 0.9μg/ml. Its inhibition on alpha-glucosidase was determined to be noncompetitive and reversible when the enzyme-substrate mixture was simultaneously treated with AEGRC as an inhibitor. In addition, when it was orally administered to rats with sucrose (2g/kg), AEGRC (250-1000mg/kg) significantly suppressed the increase of blood glucose levels after sucrose loading in a dose dependent manner. These results suggest that AEGRC might exert anti-diabetic effect by suppressing carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.

AB - The present study examined the inhibitory effect of aqueous extract from the gall of Rhus chinensis (AEGRC) on alpha-glucosidase activity, an enzyme responsible for digestion of carbohydrate to monosaccharides in the process of intestinal absorption. AEGRC inhibited Bacillus alpha-glucosidase acitvity with an IC50 of 0.9μg/ml. Its inhibition on alpha-glucosidase was determined to be noncompetitive and reversible when the enzyme-substrate mixture was simultaneously treated with AEGRC as an inhibitor. In addition, when it was orally administered to rats with sucrose (2g/kg), AEGRC (250-1000mg/kg) significantly suppressed the increase of blood glucose levels after sucrose loading in a dose dependent manner. These results suggest that AEGRC might exert anti-diabetic effect by suppressing carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.

KW - Alpha-glucosidase inhibitor

KW - Gall of Rhus chinensis

KW - Postprandial hypoglycemic effect

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U2 - 10.1016/S0378-8741(02)00370-7

DO - 10.1016/S0378-8741(02)00370-7

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AN - SCOPUS:0347927331

SN - 0378-8741

VL - 85

SP - 283

EP - 287

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

IS - 2-3

ER -

Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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