Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity

Jiyun Lee; Jieun Park; Jia Yeom; Eun Hye Han; Young Hee Lim

doi:10.1016/j.aspen.2017.03.023

Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity

Jiyun Lee, Jieun Park, Jia Yeom, Eun Hye Han, Young Hee Lim

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Maintaining a balance between blood flow and hemostasis is very important because both excessive bleeding and excessive coagulation and aggregation can cause death. In this study, the preventive effect of bee venom (BV) on blood coagulation was investigated. BV prolonged coagulation periods in a dose dependent manner compared with the negative control. It did not target any specific coagulation factor(s) but showed a broad spectrum of action against various coagulation factors. BV did not inhibit fibrinolytic activity. BV inhibited trypsin and chymotrypsin activities dose-dependently. In addition, BV did not induce bleeding disorders. In conclusion, BV plays a role in anticoagulation by inhibiting the activities of coagulation factors, which are serine proteases, not via inhibiting thrombin activity.

Original language	English
Pages (from-to)	599-604
Number of pages	6
Journal	Journal of Asia-Pacific Entomology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1016/j.aspen.2017.03.023
Publication status	Published - 2017 Jun 1

Keywords

Bee venom
Coagulation factor
Serine protease
Thrombin activity

ASJC Scopus subject areas

Insect Science

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10.1016/j.aspen.2017.03.023

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title = "Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity",

abstract = "Maintaining a balance between blood flow and hemostasis is very important because both excessive bleeding and excessive coagulation and aggregation can cause death. In this study, the preventive effect of bee venom (BV) on blood coagulation was investigated. BV prolonged coagulation periods in a dose dependent manner compared with the negative control. It did not target any specific coagulation factor(s) but showed a broad spectrum of action against various coagulation factors. BV did not inhibit fibrinolytic activity. BV inhibited trypsin and chymotrypsin activities dose-dependently. In addition, BV did not induce bleeding disorders. In conclusion, BV plays a role in anticoagulation by inhibiting the activities of coagulation factors, which are serine proteases, not via inhibiting thrombin activity.",

keywords = "Bee venom, Coagulation factor, Serine protease, Thrombin activity",

author = "Jiyun Lee and Jieun Park and Jia Yeom and Han, {Eun Hye} and Lim, {Young Hee}",

note = "Funding Information: This research was supported by the Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (114070-3). Publisher Copyright: {\textcopyright} 2017 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2017",

month = jun,

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doi = "10.1016/j.aspen.2017.03.023",

language = "English",

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T1 - Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity

AU - Lee, Jiyun

AU - Park, Jieun

AU - Yeom, Jia

AU - Han, Eun Hye

AU - Lim, Young Hee

N1 - Funding Information: This research was supported by the Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (114070-3). Publisher Copyright: © 2017 Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Maintaining a balance between blood flow and hemostasis is very important because both excessive bleeding and excessive coagulation and aggregation can cause death. In this study, the preventive effect of bee venom (BV) on blood coagulation was investigated. BV prolonged coagulation periods in a dose dependent manner compared with the negative control. It did not target any specific coagulation factor(s) but showed a broad spectrum of action against various coagulation factors. BV did not inhibit fibrinolytic activity. BV inhibited trypsin and chymotrypsin activities dose-dependently. In addition, BV did not induce bleeding disorders. In conclusion, BV plays a role in anticoagulation by inhibiting the activities of coagulation factors, which are serine proteases, not via inhibiting thrombin activity.

AB - Maintaining a balance between blood flow and hemostasis is very important because both excessive bleeding and excessive coagulation and aggregation can cause death. In this study, the preventive effect of bee venom (BV) on blood coagulation was investigated. BV prolonged coagulation periods in a dose dependent manner compared with the negative control. It did not target any specific coagulation factor(s) but showed a broad spectrum of action against various coagulation factors. BV did not inhibit fibrinolytic activity. BV inhibited trypsin and chymotrypsin activities dose-dependently. In addition, BV did not induce bleeding disorders. In conclusion, BV plays a role in anticoagulation by inhibiting the activities of coagulation factors, which are serine proteases, not via inhibiting thrombin activity.

KW - Bee venom

KW - Coagulation factor

KW - Serine protease

KW - Thrombin activity

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ER -

Inhibitory effect of bee venom on blood coagulation via anti-serine protease activity

Abstract

Keywords

ASJC Scopus subject areas

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