Abstract
Aims: The purpose of this study was to characterize the degree to which four cell lysates obtained from Lactobacillus acidophilus strains affected the cytotoxic activity of Escherichia coli O157:H7 in vitro and in vivo. Methods and Results: In a cytotoxic inhibition test that used 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide and toxin-binding ELISA assays, the activity of shiga-like toxin 2 (Stx-2) was inhibited profoundly by the cell lysates (10 mg ml-1) from two strains of L. acidophilus A4 and 30SC (>85% of survival rates compared with the control) among the five strains tested. In particular, a significant decline in the virulence level of E. coli O157:H7, under the presence of the cell lysates of L. acidophilus A4, was observed by killing assay of Caenorhabditis elegans in vivo model. Conclusions: According to our results, L. acidophilus strains might be capable of attenuating the virulence of Stx-2 produced from E. coli O157:H7. Significance and Impact of the Study: The cell lysates of L. acidophilus can be applied to a variety of foods, and can be used as adjuncts for the inhibition of Stx-2-mediated cytotoxicity.
| Original language | English |
|---|---|
| Pages (from-to) | 502-507 |
| Number of pages | 6 |
| Journal | Letters in Applied Microbiology |
| Volume | 43 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 Nov |
Keywords
- Caenorhabditis elegans
- Cytotoxicity
- Escherichia coli O157:H7
- Lactobacillus acidophilus
- Shiga-like toxin 2
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology