Injection with interleukin-4-secreting fibroblasts efficiently induces T helper type 2 cell-dominated immune response

Tae S. Kim; Seung H. Kim; Seung Y. Hwang

doi:10.1016/S0264-410X(00)00075-X

Injection with interleukin-4-secreting fibroblasts efficiently induces T helper type 2 cell-dominated immune response

Tae S. Kim, Seung H. Kim, Seung Y. Hwang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

To determine whether the paracrine secretion of interleukin-4 (IL-4) can efficiently induce T helper type 2 (Th2) cell-dominated immune response, BLK fibroblasts were stably transfected to secrete IL-4 (750 units/10⁶ cells/48 h). Their effects on T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed C57BL/6 mice, and were compared with those of free recombinant IL-4. Injection with IL-4-secreting fibroblasts (BLK/IL-4) significantly increased anti-OVA IgG1 production in OVA-primed mice. In addition, the BLK/IL-4 cells were more effective than free recombinant IL-4 in decreasing OVA-specific IFN-γ production and in increasing OVA-specific IL-4 production by splenic CD4⁺ T cells. This work suggests that IL-4- secreting fibroblasts can efficiently induce Th2 cell-dominated immune response and may be beneficial in the treatment of diseases caused by undesired Th1 cell-dominated responses. (C) 2000 Elsevier Science Ltd.

Original language	English
Pages (from-to)	2832-2837
Number of pages	6
Journal	Vaccine
Volume	18
Issue number	25
DOIs	https://doi.org/10.1016/S0264-410X(00)00075-X
Publication status	Published - 2000 Jun 15
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Drs R. Mulligan, G. Trinchieri and Y.K. Choe for providing valuable reagents. We also thank Drs I.-S. Lee and S.H. Choi for their careful review of this manuscript. This work was supported by a grant from the Korea Science and Engineering Foundation (HRC 1998 G0201 to T.S. Kim).

Keywords

Fibroblast
Interleukin-4
Ovalbumin
T helper cell

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(00)00075-X

Cite this

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title = "Injection with interleukin-4-secreting fibroblasts efficiently induces T helper type 2 cell-dominated immune response",

abstract = "To determine whether the paracrine secretion of interleukin-4 (IL-4) can efficiently induce T helper type 2 (Th2) cell-dominated immune response, BLK fibroblasts were stably transfected to secrete IL-4 (750 units/106 cells/48 h). Their effects on T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed C57BL/6 mice, and were compared with those of free recombinant IL-4. Injection with IL-4-secreting fibroblasts (BLK/IL-4) significantly increased anti-OVA IgG1 production in OVA-primed mice. In addition, the BLK/IL-4 cells were more effective than free recombinant IL-4 in decreasing OVA-specific IFN-γ production and in increasing OVA-specific IL-4 production by splenic CD4+ T cells. This work suggests that IL-4- secreting fibroblasts can efficiently induce Th2 cell-dominated immune response and may be beneficial in the treatment of diseases caused by undesired Th1 cell-dominated responses. (C) 2000 Elsevier Science Ltd.",

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note = "Funding Information: We would like to thank Drs R. Mulligan, G. Trinchieri and Y.K. Choe for providing valuable reagents. We also thank Drs I.-S. Lee and S.H. Choi for their careful review of this manuscript. This work was supported by a grant from the Korea Science and Engineering Foundation (HRC 1998 G0201 to T.S. Kim).",

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AU - Kim, Seung H.

AU - Hwang, Seung Y.

N1 - Funding Information: We would like to thank Drs R. Mulligan, G. Trinchieri and Y.K. Choe for providing valuable reagents. We also thank Drs I.-S. Lee and S.H. Choi for their careful review of this manuscript. This work was supported by a grant from the Korea Science and Engineering Foundation (HRC 1998 G0201 to T.S. Kim).

PY - 2000/6/15

Y1 - 2000/6/15

N2 - To determine whether the paracrine secretion of interleukin-4 (IL-4) can efficiently induce T helper type 2 (Th2) cell-dominated immune response, BLK fibroblasts were stably transfected to secrete IL-4 (750 units/106 cells/48 h). Their effects on T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed C57BL/6 mice, and were compared with those of free recombinant IL-4. Injection with IL-4-secreting fibroblasts (BLK/IL-4) significantly increased anti-OVA IgG1 production in OVA-primed mice. In addition, the BLK/IL-4 cells were more effective than free recombinant IL-4 in decreasing OVA-specific IFN-γ production and in increasing OVA-specific IL-4 production by splenic CD4+ T cells. This work suggests that IL-4- secreting fibroblasts can efficiently induce Th2 cell-dominated immune response and may be beneficial in the treatment of diseases caused by undesired Th1 cell-dominated responses. (C) 2000 Elsevier Science Ltd.

AB - To determine whether the paracrine secretion of interleukin-4 (IL-4) can efficiently induce T helper type 2 (Th2) cell-dominated immune response, BLK fibroblasts were stably transfected to secrete IL-4 (750 units/106 cells/48 h). Their effects on T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed C57BL/6 mice, and were compared with those of free recombinant IL-4. Injection with IL-4-secreting fibroblasts (BLK/IL-4) significantly increased anti-OVA IgG1 production in OVA-primed mice. In addition, the BLK/IL-4 cells were more effective than free recombinant IL-4 in decreasing OVA-specific IFN-γ production and in increasing OVA-specific IL-4 production by splenic CD4+ T cells. This work suggests that IL-4- secreting fibroblasts can efficiently induce Th2 cell-dominated immune response and may be beneficial in the treatment of diseases caused by undesired Th1 cell-dominated responses. (C) 2000 Elsevier Science Ltd.

KW - Fibroblast

KW - Interleukin-4

KW - Ovalbumin

KW - T helper cell

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ER -

Injection with interleukin-4-secreting fibroblasts efficiently induces T helper type 2 cell-dominated immune response

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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