To determine whether the paracrine secretion of interleukin-4 (IL-4) can efficiently induce T helper type 2 (Th2) cell-dominated immune response, BLK fibroblasts were stably transfected to secrete IL-4 (750 units/106 cells/48 h). Their effects on T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed C57BL/6 mice, and were compared with those of free recombinant IL-4. Injection with IL-4-secreting fibroblasts (BLK/IL-4) significantly increased anti-OVA IgG1 production in OVA-primed mice. In addition, the BLK/IL-4 cells were more effective than free recombinant IL-4 in decreasing OVA-specific IFN-γ production and in increasing OVA-specific IL-4 production by splenic CD4+ T cells. This work suggests that IL-4- secreting fibroblasts can efficiently induce Th2 cell-dominated immune response and may be beneficial in the treatment of diseases caused by undesired Th1 cell-dominated responses. (C) 2000 Elsevier Science Ltd.
Bibliographical noteFunding Information:
We would like to thank Drs R. Mulligan, G. Trinchieri and Y.K. Choe for providing valuable reagents. We also thank Drs I.-S. Lee and S.H. Choi for their careful review of this manuscript. This work was supported by a grant from the Korea Science and Engineering Foundation (HRC 1998 G0201 to T.S. Kim).
- T helper cell
ASJC Scopus subject areas
- Molecular Medicine
- General Immunology and Microbiology
- General Veterinary
- Public Health, Environmental and Occupational Health
- Infectious Diseases