Insights into characterization methods and biomedical applications of nanoparticle-protein corona

Yan Li, Jae Seung Lee

Research output: Contribution to journalReview articlepeer-review

26 Citations (Scopus)


Nanoparticles (NPs) exposed to a biological milieu will strongly interact with proteins, forming "coronas" on the surfaces of the NPs. The protein coronas (PCs) affect the properties of the NPs and provide a new biological identity to the particles in the biological environment. The characterization of NP-PC complexes has attracted enormous research attention, owing to the crucial effects of the properties of an NP-PC on its interactions with living systems, as well as the diverse applications of NP-PC complexes. The analysis of NP-PC complexes without a well-considered approach will inevitably lead to misunderstandings and inappropriate applications of NPs. This review introduces methods for the characterization of NP-PC complexes and investigates their recent applications in biomedicine. Furthermore, the review evaluates these characterization methods based on comprehensive critical views and provides future perspectives regarding the applications of NP-PC complexes.

Original languageEnglish
Article number3093
Issue number14
Publication statusPublished - 2020 Jul

Bibliographical note

Funding Information:
Funding: This research was supported by NRF funded by the Korean government, MSIP (NRF-2015M3A9D7031015, NRF-2018R1A1A1A05079384, and NRF-2016R1A5A1010148).

Publisher Copyright:
© 2020 by the authors.


  • Biomedical application
  • Characterization
  • Nanoparticles
  • Protein corona

ASJC Scopus subject areas

  • General Materials Science


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