TY - JOUR
T1 - Insights into growth-promoting effect of nanomaterials
T2 - Using transcriptomics and metabolomics to reveal the molecular mechanisms of MWCNTs in enhancing hyperaccumulator under heavy metal(loid)s stress
AU - Chen, Xunfeng
AU - Wang, Juncai
AU - Wang, Renyuan
AU - Zhang, Dan
AU - Chu, Shaohua
AU - Yang, Xijia
AU - Hayat, Kashif
AU - Fan, Zhengqiu
AU - Cao, Xinde
AU - Ok, Yong Sik
AU - Zhou, Pei
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 32171612 ), Shanghai " Science and Technology Innovation Action Plan " Social Development Science and Technology Project (Grant No. 20dz1204804 ), Shanghai Science and Technology Innovation Action Project (Grant No. 20392001000 ), Yunnan Expert Workstation (Grant No. 202205AF150084 ), National Key Research and Development Program (Grant No. 2016YFD0800807 ).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/10/5
Y1 - 2022/10/5
N2 - Carbon nanotubes present potential applications in soil remediation, particularly in phytoremediation. Yet, how multi-walled carbon nanotubes (MWCNTs) induced hyperaccumulator growth at molecular level remains unclear. Here, physio-biochemical, transcriptomic, and metabolomic analyses were performed to determine the effect of MWCNTs on Solanum nigrum L. (S. nigrum) growth under cadmium and arsenic stresses. 500 mg/kg MWCNTs application significantly promoted S. nigrum growth, especially for root tissues. Specially, MWCNTs application yields 1.38-fold, 1.56-fold, and 1.37-fold enhancement in the shoot length, root length, and fresh biomass, respectively. Furthermore, MWCNTs significantly strengthened P and Fe absorption in roots, as well as the activities of antioxidative enzymes. Importantly, the transcriptomic analysis indicated that S. nigrum gene expression was sensitive to MWCNTs, and MWCNTs upregulated advantageous biological processes under heavy metal(loid)s stress. Besides, MWCNTs reprogramed metabolism that related to defense system, leading to accumulation of 4-hydroxyphenylpyruvic acid (amino acid), 4-hydroxycinnamic acid (xenobiotic), and (S)-abscisic acid (lipid). In addition, key common pathways of differentially expressed metabolites and genes, including “tyrosine metabolism” and “isoquinoline alkaloid biosynthesis” were selected via integrating transcriptome and metabolome analyses. Combined omics technologies, our findings provide molecular mechanisms of MWCNTs in promoting S. nigrum growth, and highlight potential application of MWCNTs in soil remediation.
AB - Carbon nanotubes present potential applications in soil remediation, particularly in phytoremediation. Yet, how multi-walled carbon nanotubes (MWCNTs) induced hyperaccumulator growth at molecular level remains unclear. Here, physio-biochemical, transcriptomic, and metabolomic analyses were performed to determine the effect of MWCNTs on Solanum nigrum L. (S. nigrum) growth under cadmium and arsenic stresses. 500 mg/kg MWCNTs application significantly promoted S. nigrum growth, especially for root tissues. Specially, MWCNTs application yields 1.38-fold, 1.56-fold, and 1.37-fold enhancement in the shoot length, root length, and fresh biomass, respectively. Furthermore, MWCNTs significantly strengthened P and Fe absorption in roots, as well as the activities of antioxidative enzymes. Importantly, the transcriptomic analysis indicated that S. nigrum gene expression was sensitive to MWCNTs, and MWCNTs upregulated advantageous biological processes under heavy metal(loid)s stress. Besides, MWCNTs reprogramed metabolism that related to defense system, leading to accumulation of 4-hydroxyphenylpyruvic acid (amino acid), 4-hydroxycinnamic acid (xenobiotic), and (S)-abscisic acid (lipid). In addition, key common pathways of differentially expressed metabolites and genes, including “tyrosine metabolism” and “isoquinoline alkaloid biosynthesis” were selected via integrating transcriptome and metabolome analyses. Combined omics technologies, our findings provide molecular mechanisms of MWCNTs in promoting S. nigrum growth, and highlight potential application of MWCNTs in soil remediation.
KW - Cadmium and arsenic
KW - Carbon nanotubes
KW - Metabolomic analysis
KW - Solanum nigrum L.
KW - Transcriptomic analysis
UR - http://www.scopus.com/inward/record.url?scp=85134832803&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2022.129640
DO - 10.1016/j.jhazmat.2022.129640
M3 - Article
C2 - 35882170
AN - SCOPUS:85134832803
SN - 0304-3894
VL - 439
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 129640
ER -