Abstract
The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications. In the present study, two avidin-like biotin-binding proteins (named streptavidin C1 and C2) from Streptomyces cinnamonensis were newly identified while exploring antifungal proteins against Fusarium oxysporum f. sp. cucumerinum. Streptavidin C1 reveals a low correlation (a sequence identity of approximately 64%) with all known streptavidins, whereas streptavidin C2 shares a sequence identity of approximately 94% with other streptavidins. Here, the crystal structures of streptavidin C1 in the mature form and in complex with biotin at 2.1 and 2.5 Å resolution, respectively, were assessed. The overall structures present similar tetrameric features with D 2 symmetry to other (strept)avidin structures. Interestingly, the long C-terminal region comprises a short α-helix (C-Lid; residues 169-179) and an extension C-terminal peptide (ECP; residues 180-191) which stretches into the biotin-binding sites of the same monomer. This ECP sequence (-180VTSANPPAS188-) is a newly defined biotin-binding site, which reduces the ability to bind to (strept)avidin family proteins. The novel streptavidin C1 could help in the development of an engineered tetrameric streptavidin with reduced biotin-binding capacity as well as other biomaterial tools.
Original language | English |
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Pages (from-to) | 168-177 |
Number of pages | 10 |
Journal | IUCrJ |
Volume | 8 |
DOIs | |
Publication status | Published - 2021 |
Bibliographical note
Funding Information:SK was supported by grant No. 2019R1I1A1A01056 from the National Research Foundation of Korea. KYH was supported by Korea University Funds. This work was supported by project grants (Nos. 2020R1A2C2005670 and 2018M3A9F3055925) from the National Research Foundation funded by the Ministry of Science of Korea and the Next K2 project of Kyung-Nong Corp.
Publisher Copyright:
© 2021 International Union of Crystallography. All rights reserved.
Keywords
- Antifungal activity
- Biotin
- Self-binding
- Streptavidin
- Streptomyces cinnamonensis
ASJC Scopus subject areas
- Chemistry(all)
- Biochemistry
- Materials Science(all)
- Condensed Matter Physics