Insular cortex stimulation alleviates neuropathic pain through changes in the expression of collapsin response mediator protein 2 involved in synaptic plasticity

Kyeongmin Kim, Guanghai Nan, Hyeji Bak, Hee Young Kim, Junesun Kim, Myeounghoon Cha, Bae Hwan Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In recent studies, brain stimulation has shown promising potential to alleviate chronic pain. Although studies have shown that stimulation of pain-related brain regions can induce pain-relieving effects, few studies have elucidated the mechanisms of brain stimulation in the insular cortex (IC). The present study was conducted to explore the changes in characteristic molecules involved in pain modulation mechanisms and to identify the changes in synaptic plasticity after IC stimulation (ICS). Following ICS, pain-relieving behaviors and changes in proteomics were explored. Neuronal activity in the IC after ICS was observed by optical imaging. Western blotting was used to validate the proteomics data and identify the changes in the expression of glutamatergic receptors associated with synaptic plasticity. Experimental results showed that ICS effectively relieved mechanical allodynia, and proteomics identified specific changes in collapsin response mediator protein 2 (CRMP2). Neuronal activity in the neuropathic rats was significantly decreased after ICS. Neuropathic rats showed increased expression levels of phosphorylated CRMP2, alpha amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (AMPAR), and N-methyl-D-aspartate receptor (NMDAR) subunit 2B (NR2B), which were inhibited by ICS. These results indicate that ICS regulates the synaptic plasticity of ICS through pCRMP2, together with AMPAR and NR2B, to induce pain relief.

Original languageEnglish
Article number106466
JournalNeurobiology of Disease
Volume194
DOIs
Publication statusPublished - 2024 May

Bibliographical note

Publisher Copyright:
© 2023

Keywords

  • CRMP2
  • Insular cortex
  • Neuropathic pain
  • Proteomics
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neurology

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