TY - JOUR
T1 - Insulin-loaded liposomes packaged in alginate hydrogels promote the oral bioavailability of insulin
AU - Wu, Haishan
AU - Nan, Jian
AU - Yang, Liu
AU - Park, Hyun Jin
AU - Li, Jinglei
N1 - Funding Information:
This work is supported by National Natural Science Foundation of China ( 31700015 ), Fundamental Research Funds for the Central Universities ( JZ2018HGTB0244 ) and Anhui Natural Science Foundation ( 1808085QC66 ).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2023/1
Y1 - 2023/1
N2 - Compared to subcutaneous injections, oral administration of insulin would be a preferred route of drug administration for diabetic patients. For oral delivery, both liposomes and alginate hydrogels face many challenges, including early burst release of the encapsulated drug and poor intestinal drug absorption. Also, adhesion to the intestinal mucosa remains weak, which all result in a low bioavailability of the payload. This study reports on an alginate hydrogel loaded with liposomes for oral insulin administration. Liposomes (Lip) loaded with arginine-insulin complexes (AINS) were incorporated into a hydrogel prepared from cysteine modified alginate (Cys-Alg) to form liposome-in-alginate hydrogels (AINS-Lip-Gel). An ex vivo study proves that intestinal permeation of AINS and AINS-Lip is approximately 2.0 and 6.0-fold, respectively, higher than that of free insulin. The hydrogel retarded early release of insulin (∼30%) from the liposomes and enhanced the intestinal mucosal retention. In vivo experiments revealed that the AINS-Lip-Gel released insulin in a controlled manner and possessed strong hypoglycemic effects. We conclude that liposome-in-alginate hydrogels loaded with AINS represent an attractive strategy for the oral delivery of insulin.
AB - Compared to subcutaneous injections, oral administration of insulin would be a preferred route of drug administration for diabetic patients. For oral delivery, both liposomes and alginate hydrogels face many challenges, including early burst release of the encapsulated drug and poor intestinal drug absorption. Also, adhesion to the intestinal mucosa remains weak, which all result in a low bioavailability of the payload. This study reports on an alginate hydrogel loaded with liposomes for oral insulin administration. Liposomes (Lip) loaded with arginine-insulin complexes (AINS) were incorporated into a hydrogel prepared from cysteine modified alginate (Cys-Alg) to form liposome-in-alginate hydrogels (AINS-Lip-Gel). An ex vivo study proves that intestinal permeation of AINS and AINS-Lip is approximately 2.0 and 6.0-fold, respectively, higher than that of free insulin. The hydrogel retarded early release of insulin (∼30%) from the liposomes and enhanced the intestinal mucosal retention. In vivo experiments revealed that the AINS-Lip-Gel released insulin in a controlled manner and possessed strong hypoglycemic effects. We conclude that liposome-in-alginate hydrogels loaded with AINS represent an attractive strategy for the oral delivery of insulin.
KW - Alginate
KW - Cysteine
KW - Insulin
KW - Intestinal absorption
KW - Oral administration
UR - http://www.scopus.com/inward/record.url?scp=85142344789&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2022.11.032
DO - 10.1016/j.jconrel.2022.11.032
M3 - Article
C2 - 36410613
AN - SCOPUS:85142344789
SN - 0168-3659
VL - 353
SP - 51
EP - 62
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -