Insulin microcrystals prepared by the seed zone method

Hyun Kyung Lee, Jai Hyun Kwon, Sang Ha Park, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


A novel microcrystalliztion process for bovine insulin (<5 μm almost 90% in volume) was developed for pulmonary delivery using the seed zone method. The stability of bovine insulin microcrystals produced was investigated by the chromatographic analysis. The percentage of high molecular weight proteins and other insulin-related compounds of the insulin microcrystals were almost same compared with its raw material, while the percentage of A-21 desamido insulin was significantly low (P<0.001). The physicochemical properties of the bovine insulin microcrystals were measured using X-ray diffraction and differential scanning calorimetry. The physicochemical properties of the produced microcrystals were similar to those of the commercial insulin powder and milled crystalline form. Therefore, the bovine insulin microcrystals which were produced by the seed zone method maintain their physicochemical properties sufficiently good for the pulmonary delivery in terms of product-related impurities.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalJournal of Crystal Growth
Issue number2
Publication statusPublished - 2006 Aug 1

Bibliographical note

Funding Information:
Hyun-Kyung Lee hold a Brain Korea 21 fellowship from the Korean Ministry of Education & Human Resources Development.


  • A1. Microcrystal
  • A1. Pulmonary delivery
  • A1. Stability
  • A2. Seed zone method
  • B1. Insulin

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry


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