Integrating magnetic tweezers and single-molecule FRET: A comprehensive approach to studying local and global conformational changes simultaneously

Hae Jun Jung, Beom Hyeon Park, Sook Ho Kim, Seok Cheol Hong

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter presents the integration of magnetic tweezers with single-molecule FRET technology, a significant advancement in the study of nucleic acids and other biological systems. We detail the technical aspects, challenges, and current status of this hybrid technique, which combines the global manipulation and observation capabilities of magnetic tweezers with the local conformational detection of smFRET. This innovative approach enhances our ability to analyze and understand the molecular mechanics of biological systems. The chapter serves as our first formal documentation of this method, offering insights and methodologies developed in our laboratory over the past decade.

Original languageEnglish
Title of host publicationMagnetic Tweezers for the Study of Protein Structure and Function
PublisherAcademic Press Inc.
Pages167-189
Number of pages23
DOIs
Publication statusPublished - 2024 Jan

Publication series

NameMethods in Enzymology
Volume694
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Bibliographical note

Publisher Copyright:
© 2024

Keywords

  • Conformational change
  • Integration
  • Magnetic tweezers
  • Single-molecule FRET
  • Synchronization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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