Integration of electropreconcentration and electrospray ionization in a microchip

Honggu Chun

doi:10.1016/j.chroma.2018.02.037

Integration of electropreconcentration and electrospray ionization in a microchip

Honggu Chun

School of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.

Original language	English
Pages (from-to)	67-72
Number of pages	6
Journal	Journal of Chromatography A
Volume	1543
DOIs	https://doi.org/10.1016/j.chroma.2018.02.037
Publication status	Published - 2018 Mar 30

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) ( NRF-2013M3A6A4046061 , NRF-2012-0009556 , and NRF-2011-0031866 ).

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Electropreconcentration
Electrospray ionization
Ion concentration polarization
Mass spectrometry

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2018.02.037

Cite this

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title = "Integration of electropreconcentration and electrospray ionization in a microchip",

abstract = "A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.",

keywords = "Electropreconcentration, Electrospray ionization, Ion concentration polarization, Mass spectrometry",

author = "Honggu Chun",

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AU - Chun, Honggu

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) ( NRF-2013M3A6A4046061 , NRF-2012-0009556 , and NRF-2011-0031866 ). Publisher Copyright: © 2018 Elsevier B.V.

PY - 2018/3/30

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N2 - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.

AB - A microfluidic glass chip for performing sensitive mass spectrometry (MS) is developed by integrating nanochannel-based electropreconcentration with electrospray ionization (ESI). Cation analytes in acidic buffer conditions (∼pH 3) were preconcentrated at a micro-nano-microchannel junction where a positively charged polymer, polyE-323, is coated to reverse the electroosmotic flows (anodic EOF) and prevent sample adsorption. The preconcentrated cation analytes were delivered to a monolithic spray tip using an integrated field-free electrokinetic pump, enabling a stable positive ESI with negligible dead volume. The sample preconcentration-ESI-MS achieved one order of magnitude signal enhancement in MS signal with a peptide mixture.

KW - Electropreconcentration

KW - Electrospray ionization

KW - Ion concentration polarization

KW - Mass spectrometry

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DO - 10.1016/j.chroma.2018.02.037

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SN - 0021-9673

VL - 1543

SP - 67

EP - 72

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -

Integration of electropreconcentration and electrospray ionization in a microchip

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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