We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.
Bibliographical noteFunding Information:
This work was supported by Korea Science and Engineering Foundation Grant (R01-2003-000-10866-0).
Copyright 2008 Elsevier B.V., All rights reserved.
- Carbon nanotube
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering