Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

Jeongyun Kim; Juyeoul Baek; Hyughan Kim; Kyam Lee; Sanghoon Lee

doi:10.1016/j.sna.2005.12.039

Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

Jeongyun Kim, Juyeoul Baek, Hyughan Kim, Kyam Lee, Sanghoon Lee

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.

Original language	English
Pages (from-to)	7-13
Number of pages	7
Journal	Sensors and Actuators, A: Physical
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/j.sna.2005.12.039
Publication status	Published - 2006 Mar 31

Bibliographical note

Funding Information:
This work was supported by Korea Science and Engineering Foundation Grant (R01-2003-000-10866-0).

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Carbon nanotube
Glucose-detection
Microfluidics
PDMS

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering

Access to Document

10.1016/j.sna.2005.12.039

Cite this

@article{8c52354dc3484dc0ad9080065fc53bd6,

title = "Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection",

abstract = "We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.",

keywords = "Carbon nanotube, Glucose-detection, Microfluidics, PDMS",

author = "Jeongyun Kim and Juyeoul Baek and Hyughan Kim and Kyam Lee and Sanghoon Lee",

year = "2006",

month = mar,

day = "31",

doi = "10.1016/j.sna.2005.12.039",

language = "English",

volume = "128",

pages = "7--13",

journal = "Sensors and Actuators, A: Physical",

issn = "0924-4247",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

AU - Kim, Jeongyun

AU - Baek, Juyeoul

AU - Kim, Hyughan

AU - Lee, Kyam

AU - Lee, Sanghoon

PY - 2006/3/31

Y1 - 2006/3/31

N2 - We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.

AB - We propose in this paper a new, simple, and cost-effective approach to two tasks: first, the fabrication of a co-enzyme-immobilized mass of single-walled carbon nanotubes (SWNTs) that has a specific microscale shape; second, the integration of the mass into a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. An 80-μm-thick horseshoe-shaped SWNT microblock that had been physically immobilized with glucose oxidase (GOx) and horseradish peroxidase (HRP) was fabricated through the use of a 150-μm-thick flexible PDMS mold. The fabricated SWNT microblock was incorporated into the microfluidic channel for the bio-reaction on a microscale. This microfluidic device was tested for the spectroscopic glucose-detection, and the results showed that the glucose can be detected linearly (in log scale) in a wide range of glucose concentrations.

KW - Carbon nanotube

KW - Glucose-detection

KW - Microfluidics

KW - PDMS

UR - http://www.scopus.com/inward/record.url?scp=33645163291&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645163291&partnerID=8YFLogxK

U2 - 10.1016/j.sna.2005.12.039

DO - 10.1016/j.sna.2005.12.039

M3 - Article

AN - SCOPUS:33645163291

SN - 0924-4247

VL - 128

SP - 7

EP - 13

JO - Sensors and Actuators, A: Physical

JF - Sensors and Actuators, A: Physical

IS - 1

ER -

Integration of enzyme immobilized single-walled carbon nanotubes mass into the microfluidic platform and its application for the glucose-detection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this