Interactions of phenolic compounds with milk proteins

Jaejoon Han, Yoonjee Chang, Michel Britten, Daniel St-Gelais, Claude P. Champagne, Patrick Fustier, Monique Lacroix

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


This study showed that phenolic compounds mainly interacted with casein rather than whey protein. To prove this, the molecular interactions between phenolic compounds and milk proteins, such as casein and whey proteins, were investigated by measuring changes in their aggregate sizes of molecules. Size-exclusion chromatography was performed to determine the aggregate sizes of milk proteins. Results showed that when casein was mixed with an extract of green tea, grape, or cranberry, the aggregate size of casein increased as a result of chemical interactions between casein and the phenolic compounds. Meanwhile, only a negligible change in the aggregate size was observed when whey protein was mixed with phenolic compounds, implying little interaction. The higher affinity of these polyphenolic compounds with casein proteins was correlated with the high recovery potential of polyphenolic compounds in the cheese-making process as caseins are the main proteins in cheese curd. These results could help to design manufacturing processes of functional dairy products that improve yield and quality attributes.

Original languageEnglish
Pages (from-to)1881-1888
Number of pages8
JournalEuropean Food Research and Technology
Issue number9
Publication statusPublished - 2019 Sept 1

Bibliographical note

Funding Information:
We thank Dr. Claude Dupont for his very careful and insightful help to the research. This research was supported by a grant from the Fonds Québécois de Recherche sur la Nature et les Technologies (FQRNT), the Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ), Novalait, Inc. as well as Agriculture and Agri-Food Canada. Also, it was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A1B03029743).

Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.


  • Caseins
  • Molecular interactions
  • Polyphenol
  • Whey protein

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • General Chemistry
  • Biochemistry
  • Industrial and Manufacturing Engineering


Dive into the research topics of 'Interactions of phenolic compounds with milk proteins'. Together they form a unique fingerprint.

Cite this