Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee; Kwang Hee Bae; Chang Won Kho; Sunghyun Kang; Do Hee Lee; Sayeon Cho; Seongman Kang; Sang Chul Lee; Byoung Chul Park; Sung Goo Park

Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee, Kwang Hee Bae, Chang Won Kho, Sunghyun Kang, Do Hee Lee, Sayeon Cho, Seongman Kang, Sang Chul Lee, Byoung Chul Park, Sung Goo Park

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

Original language	English
Pages (from-to)	1364-1367
Number of pages	4
Journal	Journal of microbiology and biotechnology
Volume	18
Issue number	8
Publication status	Published - 2008 Aug 28

Keywords

Glutathione peroxidase 3
IP-2DE
Oxidative stress
ROS

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

@article{cde72884ed8746218229be1d9c7e2fc6,

title = "Interactome analysis of yeast glutathione peroxidase 3",

abstract = "Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.",

keywords = "Glutathione peroxidase 3, IP-2DE, Oxidative stress, ROS",

author = "Lee, {Phil Young} and Bae, {Kwang Hee} and Kho, {Chang Won} and Sunghyun Kang and Lee, {Do Hee} and Sayeon Cho and Seongman Kang and Lee, {Sang Chul} and Park, {Byoung Chul} and Park, {Sung Goo}",

year = "2008",

month = aug,

day = "28",

language = "English",

volume = "18",

pages = "1364--1367",

journal = "Journal of microbiology and biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

number = "8",

}

TY - JOUR

T1 - Interactome analysis of yeast glutathione peroxidase 3

AU - Lee, Phil Young

AU - Bae, Kwang Hee

AU - Kho, Chang Won

AU - Kang, Sunghyun

AU - Lee, Do Hee

AU - Cho, Sayeon

AU - Kang, Seongman

AU - Lee, Sang Chul

AU - Park, Byoung Chul

AU - Park, Sung Goo

PY - 2008/8/28

Y1 - 2008/8/28

N2 - Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

AB - Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

KW - Glutathione peroxidase 3

KW - IP-2DE

KW - Oxidative stress

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=56749166093&partnerID=8YFLogxK

M3 - Article

C2 - 18756095

AN - SCOPUS:56749166093

SN - 1017-7825

VL - 18

SP - 1364

EP - 1367

JO - Journal of microbiology and biotechnology

JF - Journal of microbiology and biotechnology

IS - 8

ER -

Interactome analysis of yeast glutathione peroxidase 3

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this