Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee; Kwang Hee Bae; Chang Won Kho; Sunghyun Kang; Do Hee Lee; Sayeon Cho; Seongman Kang; Sang Chul Lee; Byoung Chul Park; Sung Goo Park

Interactome analysis of yeast glutathione peroxidase 3

Phil Young Lee, Kwang Hee Bae, Chang Won Kho, Sunghyun Kang, Do Hee Lee, Sayeon Cho, Seongman Kang, Sang Chul Lee, Byoung Chul Park, Sung Goo Park

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

Original language	English
Pages (from-to)	1364-1367
Number of pages	4
Journal	Journal of microbiology and biotechnology
Volume	18
Issue number	8
Publication status	Published - 2008 Aug 28

Keywords

Glutathione peroxidase 3
IP-2DE
Oxidative stress
ROS

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

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title = "Interactome analysis of yeast glutathione peroxidase 3",

abstract = "Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.",

keywords = "Glutathione peroxidase 3, IP-2DE, Oxidative stress, ROS",

author = "Lee, {Phil Young} and Bae, {Kwang Hee} and Kho, {Chang Won} and Sunghyun Kang and Lee, {Do Hee} and Sayeon Cho and Seongman Kang and Lee, {Sang Chul} and Park, {Byoung Chul} and Park, {Sung Goo}",

year = "2008",

month = aug,

day = "28",

language = "English",

volume = "18",

pages = "1364--1367",

journal = "Journal of Microbiology and Biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

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}

TY - JOUR

T1 - Interactome analysis of yeast glutathione peroxidase 3

AU - Lee, Phil Young

AU - Bae, Kwang Hee

AU - Kho, Chang Won

AU - Kang, Sunghyun

AU - Lee, Do Hee

AU - Cho, Sayeon

AU - Kang, Seongman

AU - Lee, Sang Chul

AU - Park, Byoung Chul

AU - Park, Sung Goo

PY - 2008/8/28

Y1 - 2008/8/28

N2 - Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

AB - Oxidative stress damages all cellular constituents, and therefore, cell has to possess various defense mechanisms to cope. Saccharomyces cerevisiae, widely used as a model organism for studying cellular responses to oxidative stress, contains three glutathione peroxidase (Gpx) proteins. Among them, Gpx3 plays a major defense role against oxidative stress in S. cerevisiae. In this study, in order to identify the new interaction proteins of Gpx3, we carried out two-dimensional gel electrophoresis after immunoprecipitation (IP-2DE), and MALDI-TOF mass spectrometry. The results showed that several proteins including protein disulfide isomerase, glutaredoxin 2, and SSY protein 3 specifically interact with Gpx3. These findings led us to suggest the possibility that Gpx3, known as a redox sensor and ROS scavenger, has another functional role by interacting with several proteins with various cellular functions.

KW - Glutathione peroxidase 3

KW - IP-2DE

KW - Oxidative stress

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=56749166093&partnerID=8YFLogxK

M3 - Article

C2 - 18756095

AN - SCOPUS:56749166093

SN - 1017-7825

VL - 18

SP - 1364

EP - 1367

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 8

ER -

Interactome analysis of yeast glutathione peroxidase 3

Abstract

Keywords

ASJC Scopus subject areas

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