Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells

Ha reum Lee; Sun Young Yoon; Seok Bean Song; Yoorim Park; Tae Sung Kim; Seonghan Kim; Dae Young Hur; Hyun Keun Song; Hyunjeong Park; Daeho Cho

doi:10.1016/j.imbio.2011.04.002

Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells

Ha reum Lee, Sun Young Yoon, Seok Bean Song, Yoorim Park, Tae Sung Kim, Seonghan Kim, Dae Young Hur, Hyun Keun Song, Hyunjeong Park, Daeho Cho

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.

Original language	English
Pages (from-to)	1155-1162
Number of pages	8
Journal	Immunobiology
Volume	216
Issue number	10
DOIs	https://doi.org/10.1016/j.imbio.2011.04.002
Publication status	Published - 2011 Oct

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-314-E00092) and National Research Foundation of Korea Grant R11-2005-017-05003-0 funded by the Korean government (Ministry of Education & Science Technology).

Keywords

Interferon-gamma
Interleukin-18
Natural killer cells
Thymosin beta 4

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Hematology

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10.1016/j.imbio.2011.04.002

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title = "Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells",

abstract = "Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.",

keywords = "Interferon-gamma, Interleukin-18, Natural killer cells, Thymosin beta 4",

author = "Lee, {Ha reum} and Yoon, {Sun Young} and Song, {Seok Bean} and Yoorim Park and Kim, {Tae Sung} and Seonghan Kim and Hur, {Dae Young} and Song, {Hyun Keun} and Hyunjeong Park and Daeho Cho",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-314-E00092) and National Research Foundation of Korea Grant R11-2005-017-05003-0 funded by the Korean government (Ministry of Education & Science Technology).",

year = "2011",

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doi = "10.1016/j.imbio.2011.04.002",

language = "English",

volume = "216",

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AU - Lee, Ha reum

AU - Yoon, Sun Young

AU - Song, Seok Bean

AU - Park, Yoorim

AU - Kim, Tae Sung

AU - Kim, Seonghan

AU - Hur, Dae Young

AU - Song, Hyun Keun

AU - Park, Hyunjeong

AU - Cho, Daeho

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-314-E00092) and National Research Foundation of Korea Grant R11-2005-017-05003-0 funded by the Korean government (Ministry of Education & Science Technology).

PY - 2011/10

Y1 - 2011/10

N2 - Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.

AB - Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.

KW - Interferon-gamma

KW - Interleukin-18

KW - Natural killer cells

KW - Thymosin beta 4

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Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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