Interrogating Surface Functional Group Heterogeneity of Activated Thermoplastics Using Super-Resolution Fluorescence Microscopy

Colleen E. ONeil; Joshua M. Jackson; Sang Hee Shim; Steven A. Soper

doi:10.1021/acs.analchem.5b04472

Interrogating Surface Functional Group Heterogeneity of Activated Thermoplastics Using Super-Resolution Fluorescence Microscopy

Colleen E. ONeil, Joshua M. Jackson, Sang Hee Shim, Steven A. Soper

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We present a novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution. Thermoplastic surfaces were activated by UV/O₃ or O₂ plasma treatment under various conditions to generate pendant surface-confined carboxylic acids (-COOH). These surface functional groups were then labeled with a photoswitchable dye and interrogated using single-molecule, localization-based, super-resolution fluorescence microscopy to elucidate the surface heterogeneity of these functional groups across the activated surface. Data indicated nonuniform distributions of these functional groups for both COC and PMMA thermoplastics with the degree of heterogeneity being dose dependent. In addition, COC demonstrated relative higher surface density of functional groups compared to PMMA for both UV/O₃ and O₂ plasma treatment. The spatial distribution of -COOH groups secured from super-resolution imaging were used to simulate nonuniform patterns of electroosmotic flow in thermoplastic nanochannels. Simulations were compared to single-particle tracking of fluorescent nanoparticles within thermoplastic nanoslits to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process. (Chemical Equation Presented).

Original language	English
Pages (from-to)	3686-3696
Number of pages	11
Journal	Analytical chemistry
Volume	88
Issue number	7
DOIs	https://doi.org/10.1021/acs.analchem.5b04472
Publication status	Published - 2016 Apr 5
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank the National Institutes of Health (R21HG006278; P41EB020594), the National Science Foundation (1507577) Research Fund (1.130090.01) of Ulsan National Institute of Science and Technology, and Roche for financial support of this work. C.E.O. thanks the National Science Foundation for support through a summer fellowship program (EAPSI), and J.M.J. thanks the ACS Division of Analytical Chemistry and the Society for Analytical Chemists of Pittsburgh for funding. Furthermore, the authors thank Dr. Mateusz Hupert for his SEM studies of PMMA as well as Dr. Franklin Uba for his assistance in the construction of the single molecule electrophoresis optical system and thoughtful discussions.

Publisher Copyright:
© 2016 American Chemical Society.

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/acs.analchem.5b04472

Cite this

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title = "Interrogating Surface Functional Group Heterogeneity of Activated Thermoplastics Using Super-Resolution Fluorescence Microscopy",

abstract = "We present a novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution. Thermoplastic surfaces were activated by UV/O3 or O2 plasma treatment under various conditions to generate pendant surface-confined carboxylic acids (-COOH). These surface functional groups were then labeled with a photoswitchable dye and interrogated using single-molecule, localization-based, super-resolution fluorescence microscopy to elucidate the surface heterogeneity of these functional groups across the activated surface. Data indicated nonuniform distributions of these functional groups for both COC and PMMA thermoplastics with the degree of heterogeneity being dose dependent. In addition, COC demonstrated relative higher surface density of functional groups compared to PMMA for both UV/O3 and O2 plasma treatment. The spatial distribution of -COOH groups secured from super-resolution imaging were used to simulate nonuniform patterns of electroosmotic flow in thermoplastic nanochannels. Simulations were compared to single-particle tracking of fluorescent nanoparticles within thermoplastic nanoslits to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process. (Chemical Equation Presented).",

author = "ONeil, {Colleen E.} and Jackson, {Joshua M.} and Shim, {Sang Hee} and Soper, {Steven A.}",

note = "Funding Information: The authors would like to thank the National Institutes of Health (R21HG006278; P41EB020594), the National Science Foundation (1507577) Research Fund (1.130090.01) of Ulsan National Institute of Science and Technology, and Roche for financial support of this work. C.E.O. thanks the National Science Foundation for support through a summer fellowship program (EAPSI), and J.M.J. thanks the ACS Division of Analytical Chemistry and the Society for Analytical Chemists of Pittsburgh for funding. Furthermore, the authors thank Dr. Mateusz Hupert for his SEM studies of PMMA as well as Dr. Franklin Uba for his assistance in the construction of the single molecule electrophoresis optical system and thoughtful discussions. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

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AU - Soper, Steven A.

N1 - Funding Information: The authors would like to thank the National Institutes of Health (R21HG006278; P41EB020594), the National Science Foundation (1507577) Research Fund (1.130090.01) of Ulsan National Institute of Science and Technology, and Roche for financial support of this work. C.E.O. thanks the National Science Foundation for support through a summer fellowship program (EAPSI), and J.M.J. thanks the ACS Division of Analytical Chemistry and the Society for Analytical Chemists of Pittsburgh for funding. Furthermore, the authors thank Dr. Mateusz Hupert for his SEM studies of PMMA as well as Dr. Franklin Uba for his assistance in the construction of the single molecule electrophoresis optical system and thoughtful discussions. Publisher Copyright: © 2016 American Chemical Society.

PY - 2016/4/5

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N2 - We present a novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution. Thermoplastic surfaces were activated by UV/O3 or O2 plasma treatment under various conditions to generate pendant surface-confined carboxylic acids (-COOH). These surface functional groups were then labeled with a photoswitchable dye and interrogated using single-molecule, localization-based, super-resolution fluorescence microscopy to elucidate the surface heterogeneity of these functional groups across the activated surface. Data indicated nonuniform distributions of these functional groups for both COC and PMMA thermoplastics with the degree of heterogeneity being dose dependent. In addition, COC demonstrated relative higher surface density of functional groups compared to PMMA for both UV/O3 and O2 plasma treatment. The spatial distribution of -COOH groups secured from super-resolution imaging were used to simulate nonuniform patterns of electroosmotic flow in thermoplastic nanochannels. Simulations were compared to single-particle tracking of fluorescent nanoparticles within thermoplastic nanoslits to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process. (Chemical Equation Presented).

AB - We present a novel approach for characterizing surfaces utilizing super-resolution fluorescence microscopy with subdiffraction limit spatial resolution. Thermoplastic surfaces were activated by UV/O3 or O2 plasma treatment under various conditions to generate pendant surface-confined carboxylic acids (-COOH). These surface functional groups were then labeled with a photoswitchable dye and interrogated using single-molecule, localization-based, super-resolution fluorescence microscopy to elucidate the surface heterogeneity of these functional groups across the activated surface. Data indicated nonuniform distributions of these functional groups for both COC and PMMA thermoplastics with the degree of heterogeneity being dose dependent. In addition, COC demonstrated relative higher surface density of functional groups compared to PMMA for both UV/O3 and O2 plasma treatment. The spatial distribution of -COOH groups secured from super-resolution imaging were used to simulate nonuniform patterns of electroosmotic flow in thermoplastic nanochannels. Simulations were compared to single-particle tracking of fluorescent nanoparticles within thermoplastic nanoslits to demonstrate the effects of surface functional group heterogeneity on the electrokinetic transport process. (Chemical Equation Presented).

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Interrogating Surface Functional Group Heterogeneity of Activated Thermoplastics Using Super-Resolution Fluorescence Microscopy

Abstract

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ASJC Scopus subject areas

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