Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide

Jungyeon Kim; Joongsuk Kim; Youngsoon Um; Kyoung Heon Kim

doi:10.1016/j.procbio.2019.10.012

Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide

Jungyeon Kim, Joongsuk Kim, Youngsoon Um, Kyoung Heon Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Clostridium carboxidivorans ferments CO, CO₂, and H₂ via the Wood-Ljungdahl pathway. CO, CO_2, and H₂ are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.

Original language	English
Pages (from-to)	20-28
Number of pages	9
Journal	Process Biochemistry
Volume	89
DOIs	https://doi.org/10.1016/j.procbio.2019.10.012
Publication status	Published - 2020 Feb

Bibliographical note

Funding Information:
We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

Funding Information:
We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea ( 2016M3D3A1A01913268 ). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Appendix A

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Carbon monoxide
Clostridium carboxidivorans
Extraction solvent
Metabolomics
Optimization

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2019.10.012

Cite this

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title = "Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide",

abstract = "Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.",

keywords = "Carbon monoxide, Clostridium carboxidivorans, Extraction solvent, Metabolomics, Optimization",

author = "Jungyeon Kim and Joongsuk Kim and Youngsoon Um and Kim, {Kyoung Heon}",

note = "Funding Information: We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Funding Information: We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea ( 2016M3D3A1A01913268 ). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2020",

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doi = "10.1016/j.procbio.2019.10.012",

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AU - Kim, Jungyeon

AU - Kim, Joongsuk

AU - Um, Youngsoon

AU - Kim, Kyoung Heon

N1 - Funding Information: We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea (2016M3D3A1A01913268). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Funding Information: We would like to acknowledge the grant support from the C1 Gas Refinery Program through the National Research Foundation of Korea ( 2016M3D3A1A01913268 ). This work was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. Appendix A Publisher Copyright: © 2019 Elsevier Ltd

PY - 2020/2

Y1 - 2020/2

N2 - Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.

AB - Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for gas fermentation by C. carboxidivorans for metabolic engineering. Moreover, it is necessary to optimize the metabolite extraction solvent specifically for C. carboxidivorans fermenting syngas. In comparison with glucose media, the gas media allowed significant abundance changes of 38 and 34 metabolites in the exponential and stationary phases, respectively. Especially, C. carboxidivorans cultivated in the gas media showed changes of fatty acid metabolism and higher levels of intracellular fatty acid synthesis possibly due to cofactor imbalance and slow metabolism. Meanwhile, the evaluation of extraction solvents revealed the mixture of water-isopropanol-methanol (2:2:5, v/v/v) to be the best extraction solvent, which showed a higher extraction capability and reproducibility than pure methanol, the conventional extraction solvent. This is the first metabolomic study to demonstrate the unique intracellular metabolite profiles of the gas fermentation compared to glucose fermentation, and to evaluate water-isopropanol-methanol as the optimal metabolite extraction solvent for C. carboxidivorans on gas fermentation.

KW - Carbon monoxide

KW - Clostridium carboxidivorans

KW - Extraction solvent

KW - Metabolomics

KW - Optimization

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SN - 1359-5113

VL - 89

SP - 20

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JO - Process Biochemistry

JF - Process Biochemistry

ER -

Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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