TY - JOUR
T1 - Intramyocardial transplantation of human adipose-derived stromal cell and endothelial progenitor cell mixture was not superior to individual cell type transplantation in improving left ventricular function in rats with myocardial infarction
AU - Hong, Soon Jun
AU - Kihlken, John
AU - Choi, Seung Cheol
AU - March, Keith L.
AU - Lim, Do Sun
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2010-013-E00007).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/4/5
Y1 - 2013/4/5
N2 - Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3%, 49 ± 4%, 47 ± 4%, 39 ± 2%, P < 0.001, respectively for LVEF) (33 ± 4%, 32 ± 2%, 31 ± 2%, 23 ± 2%, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.
AB - Background: Both adipose-derived stromal cells (ASCs) and endothelial progenitor cells (EPCs) have high potential for promoting tissue revascularization and functional recovery in acute myocardial infarction (AMI) models. We investigated the functional effects of intramyocardial transplantation of a human ASC and EPC mixture in immunodeficient rats after MI. Methods: MI was induced by ligating left anterior descending coronary artery. Survived rats were randomly assigned to 1 of 4 different groups: the control group (n = 10, saline in 100 μL), the ASC group (n = 10, 106 ASCs), the EPC group (n = 10, 106 EPCs), or the ASC + EPC group (n = 10, 2 × 105 ASCs + 8 × 105 EPCs). Left ventricular (LV) function was compared using echocardiography during the 28-day follow-up. GAP43+ nerve sprouting and smooth muscle α-actin + angiogenesis were also compared. Results: Serial changes in LV ejection fraction (EF) and fractional shortening revealed significant increases in the ASC, EPC, and ASC + EPC groups when compared to the control group during the follow-up (49 ± 3%, 49 ± 4%, 47 ± 4%, 39 ± 2%, P < 0.001, respectively for LVEF) (33 ± 4%, 32 ± 2%, 31 ± 2%, 23 ± 2%, P = 0.002, respectively for fractional shortening). The number of α-actin + arterioles and GAP43+ nerve area was significantly greater in the ASC, EPC, and ASC + EPC groups when compared to the control group in the peri-infarct area (34.4 ± 1.0/mm2, 35.9 ± 1.1/mm2, 35.3 ± 0.9/mm2, 17.4 ± 0.7/mm2, P < 0.001, respectively for angiogenesis) (346.2 ± 10.7 μm2/mm 2, 357.2 ± 12.8 μm2/mm2, 368.0 ± 9.7 μm2/mm2, 174.6 ± 7.9 μm 2/mm2, P < 0.001, respectively for nerve sprouting). Conlusions: Intramyocardial injections of ASCs, EPCs, or ASCs + EPCs are effective modalities for the treatment of myocardial damage in rats and may expand the potential clinical application of ASC or EPC therapy in patients with ischemic heart disease.
KW - Adipose
KW - Angiogenesis
KW - Endothelial progenitor cell
KW - Myocardial infarction
KW - Stem cell
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U2 - 10.1016/j.ijcard.2011.06.128
DO - 10.1016/j.ijcard.2011.06.128
M3 - Article
C2 - 21794931
AN - SCOPUS:84875223679
SN - 0167-5273
VL - 164
SP - 205
EP - 211
JO - International Journal of Cardiology
JF - International Journal of Cardiology
IS - 2
ER -
