We report organically modified silica nanoparticles co-encapsulating two-photon fluorescent dye aggregates and a red-fluorescent energy acceptor, where the latter is indirectly excited through intraparticle energy transfer following two-photon excitation of the former. In vitro two-photon microscopy has demonstrated that these nanoprobes are avidly internalized into cells with bright acceptor fluorescence and that cellular optical highlighting is effected with red-to-green photoconversion by selective acceptor bleaching, while keeping the donor and the nanostructure of particles intact.
|Number of pages||7|
|Journal||Chemistry of Materials|
|Publication status||Published - 2007 Nov 13|
ASJC Scopus subject areas
- Chemical Engineering(all)
- Materials Chemistry