Investigating the in vitro osteogenic properties of the inclusion nanocarrier of icariin with beta-cyclodextrin-alginate

Somang Choi; Yeong Seok Lee; Han Saem Jo; Woong Kyo Jeong; Hak Jun Kim; Mi Hyun Song; Kyeongsoon Park; Sung Eun Kim

doi:10.3390/app10124137

Investigating the in vitro osteogenic properties of the inclusion nanocarrier of icariin with beta-cyclodextrin-alginate

Somang Choi, Yeong Seok Lee, Han Saem Jo, Woong Kyo Jeong, Hak Jun Kim, Mi Hyun Song, Kyeongsoon Park, Sung Eun Kim

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In this study, we created an inclusion nanocarrier of icariin (ICA) and β-cyclodextrin-alginate conjugate (ICA/β-CD-ALG) and determined its in vitro osteogenic ability on MC3T3-E1 cells. The morphological shape of the prepared β-CD-ALG with or without ICA was nano-sized and round. The use of β-CD-ALG achieved a sustained ICA release for up to 7 days. In vitro studies found that ICA/β-CD-ALG had a greater potential in osteogenesis on MC3T3-E1 cells compared to β-CD-ALG by exhibiting both higher alkaline phosphatase levels and the amount of calcium deposits. Moreover, ICA/β-CD-ALG greatly increased the levels of osteogenesis markers including osteocalcin (OCN) and osteopontin (OPN). Our results suggest that ICA/β-CD-ALG plays a significant role in cellular osteogenic activity.

Original language	English
Article number	4137
Journal	Applied Sciences (Switzerland)
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/app10124137
Publication status	Published - 2020 Jun 1

Keywords

Drug delivery
Icariin
MC3T3
Osteogenic activity
β-cyclodextrin-alginate

ASJC Scopus subject areas

Materials Science(all)
Instrumentation
Engineering(all)
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes

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10.3390/app10124137

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title = "Investigating the in vitro osteogenic properties of the inclusion nanocarrier of icariin with beta-cyclodextrin-alginate",

abstract = "In this study, we created an inclusion nanocarrier of icariin (ICA) and β-cyclodextrin-alginate conjugate (ICA/β-CD-ALG) and determined its in vitro osteogenic ability on MC3T3-E1 cells. The morphological shape of the prepared β-CD-ALG with or without ICA was nano-sized and round. The use of β-CD-ALG achieved a sustained ICA release for up to 7 days. In vitro studies found that ICA/β-CD-ALG had a greater potential in osteogenesis on MC3T3-E1 cells compared to β-CD-ALG by exhibiting both higher alkaline phosphatase levels and the amount of calcium deposits. Moreover, ICA/β-CD-ALG greatly increased the levels of osteogenesis markers including osteocalcin (OCN) and osteopontin (OPN). Our results suggest that ICA/β-CD-ALG plays a significant role in cellular osteogenic activity.",

keywords = "Drug delivery, Icariin, MC3T3, Osteogenic activity, β-cyclodextrin-alginate",

author = "Somang Choi and Lee, {Yeong Seok} and Jo, {Han Saem} and Jeong, {Woong Kyo} and Kim, {Hak Jun} and Song, {Mi Hyun} and Kyeongsoon Park and Kim, {Sung Eun}",

note = "Funding Information: Funding: This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Korean government, MSIP (NRF-2017M3A9B3063640) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2018R1D1A1B07050155). Publisher Copyright: {\textcopyright} 2020 by the authors.",

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doi = "10.3390/app10124137",

language = "English",

volume = "10",

journal = "Applied Sciences (Switzerland)",

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AU - Choi, Somang

AU - Lee, Yeong Seok

AU - Jo, Han Saem

AU - Jeong, Woong Kyo

AU - Kim, Hak Jun

AU - Song, Mi Hyun

AU - Park, Kyeongsoon

AU - Kim, Sung Eun

N1 - Funding Information: Funding: This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by the Korean government, MSIP (NRF-2017M3A9B3063640) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2018R1D1A1B07050155). Publisher Copyright: © 2020 by the authors.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - In this study, we created an inclusion nanocarrier of icariin (ICA) and β-cyclodextrin-alginate conjugate (ICA/β-CD-ALG) and determined its in vitro osteogenic ability on MC3T3-E1 cells. The morphological shape of the prepared β-CD-ALG with or without ICA was nano-sized and round. The use of β-CD-ALG achieved a sustained ICA release for up to 7 days. In vitro studies found that ICA/β-CD-ALG had a greater potential in osteogenesis on MC3T3-E1 cells compared to β-CD-ALG by exhibiting both higher alkaline phosphatase levels and the amount of calcium deposits. Moreover, ICA/β-CD-ALG greatly increased the levels of osteogenesis markers including osteocalcin (OCN) and osteopontin (OPN). Our results suggest that ICA/β-CD-ALG plays a significant role in cellular osteogenic activity.

AB - In this study, we created an inclusion nanocarrier of icariin (ICA) and β-cyclodextrin-alginate conjugate (ICA/β-CD-ALG) and determined its in vitro osteogenic ability on MC3T3-E1 cells. The morphological shape of the prepared β-CD-ALG with or without ICA was nano-sized and round. The use of β-CD-ALG achieved a sustained ICA release for up to 7 days. In vitro studies found that ICA/β-CD-ALG had a greater potential in osteogenesis on MC3T3-E1 cells compared to β-CD-ALG by exhibiting both higher alkaline phosphatase levels and the amount of calcium deposits. Moreover, ICA/β-CD-ALG greatly increased the levels of osteogenesis markers including osteocalcin (OCN) and osteopontin (OPN). Our results suggest that ICA/β-CD-ALG plays a significant role in cellular osteogenic activity.

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KW - Icariin

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Investigating the in vitro osteogenic properties of the inclusion nanocarrier of icariin with beta-cyclodextrin-alginate

Abstract

Keywords

ASJC Scopus subject areas

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