Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park; Deok Hie Park; Soohwan Sul; Sunghwan F. Oh; In Sook Park; Doo Soo Chung; Hie Joon Kim; Min Sik Kim; Sang Won Lee

doi:10.5012/bkcs.2004.25.12.1791

Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park, Deok Hie Park, Soohwan Sul, Sunghwan F. Oh, In Sook Park, Doo Soo Chung, Hie Joon Kim, Min Sik Kim, Sang Won Lee

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

Original language	English
Pages (from-to)	1791-1800
Number of pages	10
Journal	Bulletin of the Korean Chemical Society
Volume	25
Issue number	12
DOIs	https://doi.org/10.5012/bkcs.2004.25.12.1791
Publication status	Published - 2004 Dec 20

Keywords

Angiotensin
ESI MS/MS
Glycosylation
MALDI-TOF MS
Partial hydrolysis

ASJC Scopus subject areas

Chemistry(all)

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10.5012/bkcs.2004.25.12.1791

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title = "Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry",

abstract = "Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.",

keywords = "Angiotensin, ESI MS/MS, Glycosylation, MALDI-TOF MS, Partial hydrolysis",

author = "Park, {Soo Jin} and Park, {Deok Hie} and Soohwan Sul and Oh, {Sunghwan F.} and Park, {In Sook} and Chung, {Doo Soo} and Kim, {Hie Joon} and Kim, {Min Sik} and Lee, {Sang Won}",

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doi = "10.5012/bkcs.2004.25.12.1791",

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T1 - Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

AU - Park, Soo Jin

AU - Park, Deok Hie

AU - Sul, Soohwan

AU - Oh, Sunghwan F.

AU - Park, In Sook

AU - Chung, Doo Soo

AU - Kim, Hie Joon

AU - Kim, Min Sik

AU - Lee, Sang Won

PY - 2004/12/20

Y1 - 2004/12/20

N2 - Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

AB - Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

KW - Angiotensin

KW - ESI MS/MS

KW - Glycosylation

KW - MALDI-TOF MS

KW - Partial hydrolysis

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U2 - 10.5012/bkcs.2004.25.12.1791

DO - 10.5012/bkcs.2004.25.12.1791

M3 - Article

AN - SCOPUS:13544276937

SN - 0253-2964

VL - 25

SP - 1791

EP - 1800

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 12

ER -

Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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