Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park; Deok Hie Park; Soohwan Sul; Sunghwan F. Oh; In Sook Park; Doo Soo Chung; Hie Joon Kim; Min Sik Kim; Sang Won Lee

doi:10.5012/bkcs.2004.25.12.1791

Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Soo Jin Park
, Deok Hie Park
, Soohwan Sul
, Sunghwan F. Oh
, In Sook Park
, Doo Soo Chung
, Hie Joon Kim^*
, Min Sik Kim
, Sang Won Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

Original language	English
Pages (from-to)	1791-1800
Number of pages	10
Journal	Bulletin of the Korean Chemical Society
Volume	25
Issue number	12
DOIs	https://doi.org/10.5012/bkcs.2004.25.12.1791
Publication status	Published - 2004 Dec 20

Keywords

Angiotensin
ESI MS/MS
Glycosylation
MALDI-TOF MS
Partial hydrolysis

ASJC Scopus subject areas

General Chemistry

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10.5012/bkcs.2004.25.12.1791

Cite this

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title = "Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry",

abstract = "Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.",

keywords = "Angiotensin, ESI MS/MS, Glycosylation, MALDI-TOF MS, Partial hydrolysis",

author = "Park, \{Soo Jin\} and Park, \{Deok Hie\} and Soohwan Sul and Oh, \{Sunghwan F.\} and Park, \{In Sook\} and Chung, \{Doo Soo\} and Kim, \{Hie Joon\} and Kim, \{Min Sik\} and Lee, \{Sang Won\}",

year = "2004",

month = dec,

day = "20",

doi = "10.5012/bkcs.2004.25.12.1791",

language = "English",

volume = "25",

pages = "1791--1800",

journal = "Bulletin of the Korean Chemical Society",

issn = "0253-2964",

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number = "12",

}

TY - JOUR

T1 - Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

AU - Park, Soo Jin

AU - Park, Deok Hie

AU - Sul, Soohwan

AU - Oh, Sunghwan F.

AU - Park, In Sook

AU - Chung, Doo Soo

AU - Kim, Hie Joon

AU - Kim, Min Sik

AU - Lee, Sang Won

PY - 2004/12/20

Y1 - 2004/12/20

N2 - Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

AB - Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 °C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequencing of angiotensin by a combinatorial procedure. MALDI-TOF and electrospray ionization (ESI) tandem mass spectrometric results indicate that both the N-terminal amino group of aspartic acid and the guanidinium group of the second residue arginine are glycosylated.

KW - Angiotensin

KW - ESI MS/MS

KW - Glycosylation

KW - MALDI-TOF MS

KW - Partial hydrolysis

UR - https://www.scopus.com/pages/publications/13544276937

U2 - 10.5012/bkcs.2004.25.12.1791

DO - 10.5012/bkcs.2004.25.12.1791

M3 - Article

AN - SCOPUS:13544276937

SN - 0253-2964

VL - 25

SP - 1791

EP - 1800

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 12

ER -

Investigation of angiotensin glycosylation by MALDI-TOF and ESI tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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