Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

Hyun Hee L. Lee; Soo Yeon Chae; Myung Kook Son; Hugh I. Kim

doi:10.5478/MSL.2021.12.4.137

Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

Hyun Hee L. Lee, Soo Yeon Chae, Myung Kook Son, Hugh I. Kim

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

Original language	English
Pages (from-to)	137-145
Number of pages	9
Journal	Mass Spectrometry Letters
Volume	12
Issue number	4
DOIs	https://doi.org/10.5478/MSL.2021.12.4.137
Publication status	Published - 2021 Dec

Keywords

Glutamine
Ion chemistry
Ion mobility mass spectrometry
Tryptic peptide

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry, Genetics and Molecular Biology(all)
Spectroscopy

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10.5478/MSL.2021.12.4.137

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@article{148d411d0c9248d0b52f5c8e825460d8,

title = "Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase",

abstract = "Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.",

keywords = "Glutamine, Ion chemistry, Ion mobility mass spectrometry, Tryptic peptide",

author = "Lee, {Hyun Hee L.} and Chae, {Soo Yeon} and Son, {Myung Kook} and Kim, {Hugh I.}",

note = "Funding Information: This research was supported by a National Research Council of Science & Technology (NST) grant from the Korean Government (MSIP) (No. CAP-15-10-KRICT) and a Korea University Future Research Grant. Publisher Copyright: {\textcopyright}Korean Society for Mass Spectrometry.",

year = "2021",

month = dec,

doi = "10.5478/MSL.2021.12.4.137",

language = "English",

volume = "12",

pages = "137--145",

journal = "Mass Spectrometry Letters",

issn = "2233-4203",

publisher = "Korean Society Mass Spectrometry",

number = "4",

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TY - JOUR

T1 - Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

AU - Lee, Hyun Hee L.

AU - Chae, Soo Yeon

AU - Son, Myung Kook

AU - Kim, Hugh I.

N1 - Funding Information: This research was supported by a National Research Council of Science & Technology (NST) grant from the Korean Government (MSIP) (No. CAP-15-10-KRICT) and a Korea University Future Research Grant. Publisher Copyright: ©Korean Society for Mass Spectrometry.

PY - 2021/12

Y1 - 2021/12

N2 - Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

AB - Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

KW - Glutamine

KW - Ion chemistry

KW - Ion mobility mass spectrometry

KW - Tryptic peptide

UR - http://www.scopus.com/inward/record.url?scp=85126351214&partnerID=8YFLogxK

U2 - 10.5478/MSL.2021.12.4.137

DO - 10.5478/MSL.2021.12.4.137

M3 - Article

AN - SCOPUS:85126351214

SN - 2233-4203

VL - 12

SP - 137

EP - 145

JO - Mass Spectrometry Letters

JF - Mass Spectrometry Letters

IS - 4

ER -

Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

Abstract

Keywords

ASJC Scopus subject areas

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