Isobutanol production from empty fruit bunches

Jungwoo Yang, Jae Kyun Kim, Jung Oh Ahn, Young Ha Song, Chul Soo Shin, Yong Cheol Park, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Although isobutanol has been successfully produced from glucose-based media by various microbes, only a few studies have reported isobutanol production from lignocellulosic biomass. In this study, the feasibility of producing isobutanol from hydrothermally-pretreated empty fruit bunches (EFBs) was analyzed. Firstly, the Escherichia coli JK209 strain was constructed; subsequently, optimal fermentation conditions for isobutanol production using this strain were determined. Next, the raw EFBs were hydrothermally pretreated at 190 °C for 15 min, and the pretreated EFBs were hydrolyzed using 10 FPU of cellulase/g glucan. Finally, the resulting hydrolysate was fermented at 25 °C for 84 h, and isobutanol give rise to the yield of 0.26 g isobutanol/g glucose. These results imply that 80.1 g of isobutanol could be produced from 1 kg dry weight of EFBs. To our knowledge, this is the first feasibility study of isobutanol production from pretreated EFBs.

Original languageEnglish
Pages (from-to)1124-1130
Number of pages7
JournalRenewable Energy
Volume157
DOIs
Publication statusPublished - 2020 Sept

Bibliographical note

Funding Information:
This work was supported by the Mid-career Researcher Program (2020R1A2B5B02002631) and the C1 Gas Refinery Program (2016M3D3A1A01913268), both through the National Research Foundation of Korea. We acknowledge the facility support of the Institute of Biomedical and Food Safety at CJ Food Safety Hall, Korea University.

Funding Information:
This work was supported by the Mid-career Researcher Program ( 2020R1A2B5B02002631 ) and the C1 Gas Refinery Program ( 2016M3D3A1A01913268 ), both through the National Research Foundation of Korea . We acknowledge the facility support of the Institute of Biomedical and Food Safety at CJ Food Safety Hall, Korea University .

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

  • Empty fruit bunches
  • Isobutanol
  • Lignocellulose
  • Pretreatment
  • Separate hydrolysis and fermentation

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

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