Abstract
Isoflavones identified as inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in soybean paste were assayed using the catalytic portion of Syrian hamster HMG-CoA reductase, and the kinetic values were measured using HMG-CoA and NADPH. The inhibition of HMG-CoA reductase by these inhibitors was competitive with HMG-CoA and noncompetitive with NADPH. Ki values for genistein, daidzein, and glycitein were 27.7, 49.5, and 94.7 μM, respectively.
Original language | English |
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Pages (from-to) | 428-432 |
Number of pages | 5 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 68 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 Feb |
Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by a grant from the National Research Laboratory Program funded by the Ministry of Science and Technology of the Republic of Korea.
Keywords
- Cholesterol
- Genistein
- HMG-CoA reductase
- Isoflavone
- Soybean
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry