TY - JOUR
T1 - Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China
AU - Yu, Jicheng
AU - Zhang, Liu
AU - Liu, Qiu
AU - Qi, Xiaohui
AU - Ji, Ying
AU - Kim, Beom Seok
N1 - Funding Information:
This research was financially funded by the National Natural Science Foundation of China (No. 31270057, No. 21276047 and No. 31070005), joint research projects supported by the National Natural Science Foundation of China and Korea National Research Foundation (No. 31311140255 and No. 2013K2A2A2000535), the Fundamental Research Funds for the Central Universities (DC201501020201).
Publisher Copyright:
© 2015 Hainan Medical University.
PY - 2015
Y1 - 2015
N2 - Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemical, and physiological characteristics. Methods: Eight different production media were used to isolate actinomycetes from different stations of marine soil sediments in Yalujiang coastal wetland and the genotypic positions were established by 16S rDNA. Results: A total of 172 actinomycetal isolates were obtained from 13 samples using five media. The most effective culture media in the isolation of actinobacteria were Gause's Synthetic agar and Starch-casein agar. Among 172 isolates, 46 isolates (26.74%) showed antibacterial activity, 70.93% belonged to the genus Streptomyces, others were Micromonospora spp. and Rhodococcus spp. Out of the 46 isolates, two cultures were further supported by morphological characterization analysis. Conclusions: This is the first report about actinomycetes isolated from Yalujiang coastal wetland and it seems that the promising isolates from the unusual/unexplored wetland may prove to be an important step in the development of microbial natural product research.
AB - Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemical, and physiological characteristics. Methods: Eight different production media were used to isolate actinomycetes from different stations of marine soil sediments in Yalujiang coastal wetland and the genotypic positions were established by 16S rDNA. Results: A total of 172 actinomycetal isolates were obtained from 13 samples using five media. The most effective culture media in the isolation of actinobacteria were Gause's Synthetic agar and Starch-casein agar. Among 172 isolates, 46 isolates (26.74%) showed antibacterial activity, 70.93% belonged to the genus Streptomyces, others were Micromonospora spp. and Rhodococcus spp. Out of the 46 isolates, two cultures were further supported by morphological characterization analysis. Conclusions: This is the first report about actinomycetes isolated from Yalujiang coastal wetland and it seems that the promising isolates from the unusual/unexplored wetland may prove to be an important step in the development of microbial natural product research.
KW - 16S rRNA
KW - Actinomycetes
KW - Antimicrobial activity
KW - Yalujiang coastal wetland
UR - http://www.scopus.com/inward/record.url?scp=84942981577&partnerID=8YFLogxK
U2 - 10.1016/j.apjtb.2015.04.007
DO - 10.1016/j.apjtb.2015.04.007
M3 - Article
AN - SCOPUS:84942981577
SN - 2221-1691
VL - 5
SP - 555
EP - 560
JO - Asian Pacific Journal of Tropical Biomedicine
JF - Asian Pacific Journal of Tropical Biomedicine
IS - 7
ER -
