TY - JOUR
T1 - Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells
AU - Moon, Jai Hee
AU - Kwak, Sung Sik
AU - Park, Gyuman
AU - Jung, Hye Youn
AU - Yoon, Byung Sun
AU - Park, Jaeyeo
AU - Ryu, Kyung Su
AU - Choi, Seung Cheol
AU - Maeng, Isaac
AU - Kim, Bona
AU - Jun, Eun Kyung
AU - Kim, Soonseong
AU - Kim, Aeree
AU - Oh, Sejong
AU - Kim, Hyunggee
AU - Kim, Ki Dong
AU - You, Seungkwon
PY - 2008/8
Y1 - 2008/8
N2 - In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.
AB - In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.
UR - http://www.scopus.com/inward/record.url?scp=51849133670&partnerID=8YFLogxK
U2 - 10.1089/scd.2007.0210
DO - 10.1089/scd.2007.0210
M3 - Article
C2 - 18710345
AN - SCOPUS:51849133670
SN - 1547-3287
VL - 17
SP - 713
EP - 724
JO - Stem cells and development
JF - Stem cells and development
IS - 4
ER -