Isolation and identification of extracellular matrix proteins from oil-based CASPERized mouse brains for matrisomal analysis

Byung Geun Ha, Yu Jin Jang, Eun Soo Lee, Byung Gyu Kim, Kyungjae Myung, Woong Sun, Sung Jin Jeong

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The extracellular matrix (ECM) components present within all tissues and organs help to maintain the cytoskeletal architecture and tissue morphology. Although the ECM plays a role in cellular events and signaling pathways, it has not been well studied due its insolubility and complexity. Brain tissue has a higher cell density and weaker mechanical strength than other tissues in the body. When removing cells using a general decellularization method to produce scaffolds and obtain ECM proteins, various problems must be considered because tissues are easily damaged. To retain the brain shape and ECM components, we performed decellularization in combination with polymerization. We immersed mouse brains in oil for polymerization and decellularization via O-CASPER (Oil-based Clinically and Experimentally Applicable Acellular Tissue Scaffold Production for Tissue Engineering and Regenerative Medicine) and then isolated ECM components using sequential matrisome preparation reagents (SMPRs), namely, RIPA, PNGase F, and concanavalin A. Adult mouse brains were preserved with our decellularization method. Western blot and LC-MS/MS analyses revealed that ECM components, including collagen and laminin, were isolated efficiently from decellularized mouse brains using SMPRs. Our method will be useful to obtain matrisomal data and perform functional studies using adult mouse brains and other tissues.

Original languageEnglish
Article numbere14777
Issue number4
Publication statusPublished - 2023 Apr

Bibliographical note

Funding Information:
This work was supported by KBRI [22-BR-02-01]; the National Research Foundation of Korea [ NRF-2015M3C7A1029037 and 2017M3C7A104809 2].

Publisher Copyright:
© 2023


  • Decellularization
  • Extracellular matrix
  • LC-MS/MS
  • Matrisome
  • Tissue engineering

ASJC Scopus subject areas

  • General


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