Isolation of a phytase-producing Bacillus sp. KHU-10 and its phytase production

Yang Mun Choi, Dong Ouk Noh, Sung Ho Cho, Hyo Ku Lee, Hyung Joo Suh, Soo Hyun Chung

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27 Citations (Scopus)


A bacterial strain producing high level of an extracellular phytase was isolated from cooked rice and identified as a strain of Bacillus sp. and designated as Bacillus sp. KHU-10. Optimum culture conditions were investigated for the maximum productivity of phytase by Bacillus sp. KHU-10. 1.0% Maltose and 1.0% peptone with 0.5% beef extract were the best carbon source and nitrogen source, respectively. The addition of CaCl2 stimulated the enzyme productivity with concentration between 0.01% and 0.2%, in the medium. Although sodium phosphate increased the cell mass, the enzyme activity decreased. Calcium phytate and wheat bran containing phytate did not enhance the enzyme production. Under the optimum medium, the production of the phytase reached the highest level of 0.2 unit/ml after 4 days of incubation.

Original languageEnglish
Pages (from-to)223-226
Number of pages4
JournalJournal of microbiology and biotechnology
Issue number2
Publication statusPublished - 1999 Apr


  • Bacillus sp. KHU-10
  • Phytase
  • Phytic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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