Abstract
The aim of this study was to identify sleep-promoting substance from Polygonatum sibiricum rhizome extract (PSE) with the regulation of sleep architecture. PSE showed a decrease in sleep latency time and an increase in the sleeping time. In the electroencephalography analysis of rats, PSE (150 mg/kg) showed an increase of non-rapid eye movement by 38% and a decrease of rapid eye movement by 31% compared to the control. This sleep-promoting activity was found to be involved in the GABAA-BDZ receptor. The chemical structure of the pure compound was determined by the 1H and 13C nuclear magnetic resonance spectroscopy and gas chromatography mass spectrometry analysis; active compound was glyceryl-1-monolinoleate. The commercial standard glyceryl-1-monolinoleate showed a similar inhibitory concentration on [3H]-flumazenil binding to GABAA-BDZ receptors with final active fraction of PSE. The results indicate that glyceryl-1-monolinoleate is a major active compound responsible for the PSE-derived sleep promotion.
Original language | English |
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Pages (from-to) | 1833-1842 |
Number of pages | 10 |
Journal | Food Science and Biotechnology |
Volume | 27 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2018 Dec 1 |
Keywords
- GABA type A-benzodiazepine receptor
- Glyceryl monolinoleate
- Insomnia
- Polygonatum sibiricum
- Sleep
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology