Isolation of extracellular vesicles from small volumes of plasma using a microfluidic aqueous two-phase system

Bo Hoon Han, Sumi Kim, Geeyoon Seo, Youhee Heo, Seok Chung, Ji Yoon Kang

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

As conventional bulky methods for extracellular vesicle (EV) separation are unsuitable for small volumes of samples, microfluidic devices are thought to offer a solution for the integrated and automatic processing of EV separation. This study demonstrates a simple microfluidic aqueous two-phase system (ATPS) for EV separation with high recovery efficiency to overcome the limitation of previous devices, which require complex external equipment or high cost manufacturing. With polyethylene glycol and dextran in the microfluidic channel, the isolation mechanism of the microfluidic ATPS was analyzed by comparison between two-phase and one-phase systems. Our device could facilitate continuous EV isolation with 83.4% recovery efficiency and remove 65.4% of the proteins from the EV-protein mixture. EVs were also successfully isolated from human plasma at high recovery efficiency.

Original languageEnglish
Pages (from-to)3552-3559
Number of pages8
JournalLab on a Chip
Volume20
Issue number19
DOIs
Publication statusPublished - 2020 Oct 7

Bibliographical note

Funding Information:
This research was supported mainly by the Nano-material Technology Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017M3A7B4049851). It was also supported by the Internal Fund of the Korea Institute of Science and Technology (2E29221 and 2E29200) as well as by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI14C3319).

Publisher Copyright:
© The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering

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