Isolation of spheroid-forming single cells from gastric cancer cell lines: Enrichment of cancer stem-like cells

Jong Won Lee, Jae Sook Sung, Young Soo Park, Seok Chung, Yeul Hong Kim

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Isolation of spheroid-forming cells is important to investigate cancer stem cell (CSC) characteristics. However, conventional tumor spheroid culture methods have not proven suitable because the aggregated spheroids generally maintain their original heterogeneity and harbor multiple cells with various characteristics. Here we cultured spheroids using a polydimethylsiloxane microwell-based method and a limiting dilution protocol. We then isolated and enriched for CSCs that formed single cell-derived spheroids from gastric cancer cell lines. Cells from the microwell demonstrated higher self-renewal, increased expression of stem cell markers and resistance to apoptosis compared with spheroid cells made by the traditional method. This novel approach allows efficient cancer stem cell isolation and represents a step forward in cancer stem cell studies.

    Original languageEnglish
    Pages (from-to)197-203
    Number of pages7
    JournalBioTechniques
    Volume65
    Issue number4
    DOIs
    Publication statusPublished - 2018 Oct

    Bibliographical note

    Publisher Copyright:
    © 2018 Future Science. All Rights Reserved.

    Keywords

    • Cancer stem cells
    • Gastric cancer
    • Spheroids

    ASJC Scopus subject areas

    • Biotechnology
    • General Biochemistry,Genetics and Molecular Biology

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