Jones Matrix Microscopy for Living Eukaryotic Cells

Kwanjun Park, Taeseok Daniel Yang, Dongkwon Seo, Min Gyu Hyeon, Taedong Kong, Beop Min Kim, Yeonho Choi, Wonshik Choi, Youngwoon Choi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Polarization of light carries important information regarding the materials included by biological samples. A Jones matrix is a general tool for quantifying the degree of polarization, however, its measurement has been limited mostly to connective tissues with strong polarization response due to the lack of measurement sensitivity. Here, we demonstrate polarization phase microscopy capable of measuring a Jones matrix of a living eukaryotic cell. Our strategy combines synthetic aperture imaging with polarization phase microscopy to improve the polarization sensitivity. The resultant suppression of intrinsic phase noise in the measurement allows a Jones matrix of a single eukaryotic cell to be clearly visualized. Using the synthesized Jones matrices, the characteristic cellular polarization properties of normal and cancer cells were quantified, and substantial differences between the two kinds were quantitatively identified.

Original languageEnglish
Pages (from-to)3042-3050
Number of pages9
JournalACS Photonics
Issue number10
Publication statusPublished - 2021 Oct 20

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean government (MSIT; 2021R1A2C2012069, 2019H1A2A1076334, 2017R1A6A3A11031083, 2021R1I1A1A01059752, 2020R1I1A1A01074864) and the Institute for Basic Science (IBS-R023-D1). It was also supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (HI14C3477).

Publisher Copyright:
© 2021 American Chemical Society.


  • cancer cell classification
  • lipid droplet
  • phase retardation
  • polarization phase microscopy
  • single cell Jones matrix
  • synthetic aperture imaging

ASJC Scopus subject areas

  • Biotechnology
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering


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