Kappa-carrageenan-based dual crosslinkable bioink for extrusion type bioprinting

Wonseop Lim, Gyeong Jin Kim, Hyun Woo Kim, Jiyeon Lee, Xiaowei Zhang, Min Gyeong Kang, Jeong Wook Seo, Jae Min Cha, Hyun Jin Park, Min Young Lee, Su Ryon Shin, Seon Young Shin, Hojae Bae

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


Bioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability of the bioink depends on the physical properties such as viscosity. However, the high viscosity bioink puts shear stress on the cells and low viscosity bioink cannot maintain complex tissue structure firmly after the printing. In this work, we applied dual crosslinkable bioink using Kappa-carrageenan (κ-CA) to overcome existing shortcomings. κ-CA has properties such as biocompatibility, biodegradability, shear-thinning and ionic gelation but the difficulty of controlling gelation properties makes it unsuitable for application in 3D bioprinting. This problem was solved by synthesizing methacrylated Kappa-carrageenan (MA-κ-CA), which can be dual crosslinked through ionic and UV (Ultraviolet) crosslinking to form hydrogel using NIH-3T3 cells. Through MA substitutions, the rheological properties of the gel could be controlled to reduce the shear stress. Moreover, bioprinting using the cell-laden MA-κ-CA showed cell compatibility with enhanced shape retention capability. The potential to control the physical properties through dual crosslinking of MA-κ-CA hydrogel is expected to be widely applied in 3D bioprinting applications.

Original languageEnglish
Article number2377
Pages (from-to)1-15
Number of pages15
Issue number10
Publication statusPublished - 2020 Oct

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • 3D bioprinting
  • Dual crosslinking hydrogel
  • Kappa-carrageenan
  • Methacrylic anhydride

ASJC Scopus subject areas

  • General Chemistry
  • Polymers and Plastics


Dive into the research topics of 'Kappa-carrageenan-based dual crosslinkable bioink for extrusion type bioprinting'. Together they form a unique fingerprint.

Cite this