Keratinocyte-specific knockout mice models via Cre–loxP recombination system

Ji Won Son, Jung Jin Shin, Min Gyu Kim, Jaehyung Kim, Sang Wook Son

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


Purpose of review: The Cre recombinase/loxP (Cre/loxP) system has emerged as a useful tool in genetic manipulations. Generally, any DNA sequence of interest can be deleted when flanked with loxP sites. The conditional (timely or spatially controlled) expression of Cre recombinase enables to determine, where (e.g. in which cell type or tissue) and when (at which time of life or of developmental stage of cells/tissues) the deletion of the floxed DNA sequence should occur. Therefore, two mouse lines are usually needed for conditional site-specific genome modification. This review aims to provide a general overview of the Cre/loxP system, elaborate on the different keratinocyte specific knockout mice used to study gene function in keratinocytes, and finally demonstrate the generation of a conditional knockout mice. Recent findings: Knockout mice have been efficient and powerful approach to understand the functions of specific genes in development and physiological homeostasis. However, deletion of the specific genes which are concerned in stages of development induces to embryonic lethality. To overcome this obstacle, Cre/loxP system has been used to delete a gene in a specific organ or tissue, or at a specific embryonic developmental stage. Conditional knockout mice have allowed extensive research of the epidermis and helped elucidate roles of many gene functions in keratinocytes.

Original languageEnglish
Pages (from-to)15-27
Number of pages13
JournalMolecular and Cellular Toxicology
Issue number1
Publication statusPublished - 2021 Jan

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (NRF-2019R1A2B5B01070162).

Publisher Copyright:
© 2020, The Korean Society of Toxicogenomics and Toxicoproteomics.


  • Cre/loxP
  • Epidermis
  • Floxed
  • Keratinocyte
  • Knockout mouse
  • STAT3

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


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