Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa: Eco-friendly production and specific activity of deinoxanthins

Wonjae Kim; Minkyung Kim; Minyoung Hong; Woojun Park

doi:10.1016/j.envres.2021.111455

Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa: Eco-friendly production and specific activity of deinoxanthins

Wonjae Kim
, Minkyung Kim
, Minyoung Hong
, Woojun Park^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Cyanobacterial blooms caused mainly by Microcystis aeruginosa could be controlled using chemical and biological agents such as H₂O₂, antagonistic bacteria, and enzymes. Little is known about the possible toxic effects of bacterial membrane pigments on M. aeruginosa cells. Deinococcus metallilatus MA1002 cultured under light increased the production of several carotenoid-like compounds by upregulating two deinoxanthin biosynthesis genes: crtO and cruC. The deinoxanthin compounds were identified using thin-layer chromatography, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. D. metallilatus was cultured with agricultural by-products under light to produce the deinoxanthin compounds. Soybean meal, from six tested agricultural by-products, was selected as the single factor for making an economical medium to produce deinoxanthin compounds. The growth of axenic M. aeruginosa PCC7806, as well as other xenic cyanobacteria such as Cyanobium gracile, Trichormus variabilis, and Dolichospermum circinale, were inhibited by the deinoxanthin compounds. Scanning electron microscopic images showed the complete collapse of M. aeruginosa cells under deinoxanthin treatment, probably due to its interference with cyanobacterial membrane synthesis during cellular elongation. Deinoxanthins appeared to be nontoxic to other non-cyanobacteria such as Acinetobacter, Pseudomonas, Methylobacterium, and Bacillus species, suggesting that it can be a novel candidate for preventing cyanobacterial blooms through its specific activity against cyanobacteria.

Original language	English
Article number	111455
Journal	Environmental Research
Volume	200
DOIs	https://doi.org/10.1016/j.envres.2021.111455
Publication status	Published - 2021 Sept

Bibliographical note

Funding Information:
This work was supported by grants from the Korea University [ K2006821 ] and the National Institute of Biological Resources (NIBR) , funded by the Ministry of Environment (MOE) of the Republic of Korea [ NIBR202123201 ]. Two algal species (Chlorella vulgaris UTEX 2714 and Euglena gracilis strain Z) were provided by laboratory of biomass utilization at Korea University.

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

Agricultural by-product
Antibacterial compounds
Carotenoid
Deinoxanthin derivative
Pigment
Soybean meal

ASJC Scopus subject areas

Biochemistry
General Environmental Science

Access to Document

10.1016/j.envres.2021.111455

Cite this

@article{3618aa3a8a8648e5ba1d117f0e8dcb09,

title = "Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa: Eco-friendly production and specific activity of deinoxanthins",

abstract = "Cyanobacterial blooms caused mainly by Microcystis aeruginosa could be controlled using chemical and biological agents such as H2O2, antagonistic bacteria, and enzymes. Little is known about the possible toxic effects of bacterial membrane pigments on M. aeruginosa cells. Deinococcus metallilatus MA1002 cultured under light increased the production of several carotenoid-like compounds by upregulating two deinoxanthin biosynthesis genes: crtO and cruC. The deinoxanthin compounds were identified using thin-layer chromatography, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. D. metallilatus was cultured with agricultural by-products under light to produce the deinoxanthin compounds. Soybean meal, from six tested agricultural by-products, was selected as the single factor for making an economical medium to produce deinoxanthin compounds. The growth of axenic M. aeruginosa PCC7806, as well as other xenic cyanobacteria such as Cyanobium gracile, Trichormus variabilis, and Dolichospermum circinale, were inhibited by the deinoxanthin compounds. Scanning electron microscopic images showed the complete collapse of M. aeruginosa cells under deinoxanthin treatment, probably due to its interference with cyanobacterial membrane synthesis during cellular elongation. Deinoxanthins appeared to be nontoxic to other non-cyanobacteria such as Acinetobacter, Pseudomonas, Methylobacterium, and Bacillus species, suggesting that it can be a novel candidate for preventing cyanobacterial blooms through its specific activity against cyanobacteria.",

keywords = "Agricultural by-product, Antibacterial compounds, Carotenoid, Deinoxanthin derivative, Pigment, Soybean meal",

author = "Wonjae Kim and Minkyung Kim and Minyoung Hong and Woojun Park",

note = "Funding Information: This work was supported by grants from the Korea University [ K2006821 ] and the National Institute of Biological Resources (NIBR) , funded by the Ministry of Environment (MOE) of the Republic of Korea [ NIBR202123201 ]. Two algal species (Chlorella vulgaris UTEX 2714 and Euglena gracilis strain Z) were provided by laboratory of biomass utilization at Korea University. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = sep,

doi = "10.1016/j.envres.2021.111455",

language = "English",

volume = "200",

journal = "Environmental Research",

issn = "0013-9351",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa

T2 - Eco-friendly production and specific activity of deinoxanthins

AU - Kim, Wonjae

AU - Kim, Minkyung

AU - Hong, Minyoung

AU - Park, Woojun

N1 - Funding Information: This work was supported by grants from the Korea University [ K2006821 ] and the National Institute of Biological Resources (NIBR) , funded by the Ministry of Environment (MOE) of the Republic of Korea [ NIBR202123201 ]. Two algal species (Chlorella vulgaris UTEX 2714 and Euglena gracilis strain Z) were provided by laboratory of biomass utilization at Korea University. Publisher Copyright: © 2021 Elsevier Inc.

PY - 2021/9

Y1 - 2021/9

N2 - Cyanobacterial blooms caused mainly by Microcystis aeruginosa could be controlled using chemical and biological agents such as H2O2, antagonistic bacteria, and enzymes. Little is known about the possible toxic effects of bacterial membrane pigments on M. aeruginosa cells. Deinococcus metallilatus MA1002 cultured under light increased the production of several carotenoid-like compounds by upregulating two deinoxanthin biosynthesis genes: crtO and cruC. The deinoxanthin compounds were identified using thin-layer chromatography, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. D. metallilatus was cultured with agricultural by-products under light to produce the deinoxanthin compounds. Soybean meal, from six tested agricultural by-products, was selected as the single factor for making an economical medium to produce deinoxanthin compounds. The growth of axenic M. aeruginosa PCC7806, as well as other xenic cyanobacteria such as Cyanobium gracile, Trichormus variabilis, and Dolichospermum circinale, were inhibited by the deinoxanthin compounds. Scanning electron microscopic images showed the complete collapse of M. aeruginosa cells under deinoxanthin treatment, probably due to its interference with cyanobacterial membrane synthesis during cellular elongation. Deinoxanthins appeared to be nontoxic to other non-cyanobacteria such as Acinetobacter, Pseudomonas, Methylobacterium, and Bacillus species, suggesting that it can be a novel candidate for preventing cyanobacterial blooms through its specific activity against cyanobacteria.

AB - Cyanobacterial blooms caused mainly by Microcystis aeruginosa could be controlled using chemical and biological agents such as H2O2, antagonistic bacteria, and enzymes. Little is known about the possible toxic effects of bacterial membrane pigments on M. aeruginosa cells. Deinococcus metallilatus MA1002 cultured under light increased the production of several carotenoid-like compounds by upregulating two deinoxanthin biosynthesis genes: crtO and cruC. The deinoxanthin compounds were identified using thin-layer chromatography, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. D. metallilatus was cultured with agricultural by-products under light to produce the deinoxanthin compounds. Soybean meal, from six tested agricultural by-products, was selected as the single factor for making an economical medium to produce deinoxanthin compounds. The growth of axenic M. aeruginosa PCC7806, as well as other xenic cyanobacteria such as Cyanobium gracile, Trichormus variabilis, and Dolichospermum circinale, were inhibited by the deinoxanthin compounds. Scanning electron microscopic images showed the complete collapse of M. aeruginosa cells under deinoxanthin treatment, probably due to its interference with cyanobacterial membrane synthesis during cellular elongation. Deinoxanthins appeared to be nontoxic to other non-cyanobacteria such as Acinetobacter, Pseudomonas, Methylobacterium, and Bacillus species, suggesting that it can be a novel candidate for preventing cyanobacterial blooms through its specific activity against cyanobacteria.

KW - Agricultural by-product

KW - Antibacterial compounds

KW - Carotenoid

KW - Deinoxanthin derivative

KW - Pigment

KW - Soybean meal

UR - https://www.scopus.com/pages/publications/85107806103

U2 - 10.1016/j.envres.2021.111455

DO - 10.1016/j.envres.2021.111455

M3 - Article

C2 - 34118245

AN - SCOPUS:85107806103

SN - 0013-9351

VL - 200

JO - Environmental Research

JF - Environmental Research

M1 - 111455

ER -

Killing effect of deinoxanthins on cyanobloom-forming Microcystis aeruginosa: Eco-friendly production and specific activity of deinoxanthins

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this