Kinase gene fusions in defined subsets of melanoma

Jacqueline Turner; Kasey Couts; Jamie Sheren; Siriwimon Saichaemchan; Witthawat Ariyawutyakorn; Izabela Avolio; Ethan Cabral; Magdelena Glogowska; Carol Amato; Steven Robinson; Jennifer Hintzsche; Allison Applegate; Eric Seelenfreund; Rita Gonzalez; Keith Wells; Stacey Bagby; John Tentler; Aik Choon Tan; Joshua Wisell; Marileila Varella-Garcia; William Robinson

doi:10.1111/pcmr.12560

Kinase gene fusions in defined subsets of melanoma

Jacqueline Turner, Kasey Couts, Jamie Sheren, Siriwimon Saichaemchan, Witthawat Ariyawutyakorn, Izabela Avolio, Ethan Cabral, Magdelena Glogowska, Carol Amato, Steven Robinson, Jennifer Hintzsche, Allison Applegate, Eric Seelenfreund, Rita Gonzalez, Keith Wells, Stacey Bagby, John Tentler, Aik Choon Tan, Joshua Wisell, Marileila Varella-GarciaWilliam Robinson

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break-apart fluorescence in situ hybridization (FISH) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF, RET, and ROS1. Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC10-BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.

Original language	English
Pages (from-to)	53-62
Number of pages	10
Journal	Pigment Cell and Melanoma Research
Volume	30
Issue number	1
DOIs	https://doi.org/10.1111/pcmr.12560
Publication status	Published - 2017 Jan 1

Bibliographical note

Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

acral
kinase
melanoma
pan-negative
rearrangement

ASJC Scopus subject areas

Oncology
General Biochemistry,Genetics and Molecular Biology
Dermatology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/pcmr.12560

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Turner, J., Couts, K., Sheren, J., Saichaemchan, S., Ariyawutyakorn, W., Avolio, I., Cabral, E., Glogowska, M., Amato, C., Robinson, S., Hintzsche, J., Applegate, A., Seelenfreund, E., Gonzalez, R., Wells, K., Bagby, S., Tentler, J., Tan, A. C., Wisell, J., ... Robinson, W. (2017). Kinase gene fusions in defined subsets of melanoma. Pigment Cell and Melanoma Research, 30(1), 53-62. https://doi.org/10.1111/pcmr.12560

Turner, J, Couts, K, Sheren, J, Saichaemchan, S, Ariyawutyakorn, W, Avolio, I, Cabral, E, Glogowska, M, Amato, C, Robinson, S, Hintzsche, J, Applegate, A, Seelenfreund, E, Gonzalez, R, Wells, K, Bagby, S, Tentler, J, Tan, AC, Wisell, J, Varella-Garcia, M & Robinson, W 2017, 'Kinase gene fusions in defined subsets of melanoma', Pigment Cell and Melanoma Research, vol. 30, no. 1, pp. 53-62. https://doi.org/10.1111/pcmr.12560

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abstract = "Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break-apart fluorescence in situ hybridization (FISH) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF, RET, and ROS1. Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC10-BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.",

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AU - Turner, Jacqueline

AU - Couts, Kasey

AU - Sheren, Jamie

AU - Saichaemchan, Siriwimon

AU - Ariyawutyakorn, Witthawat

AU - Avolio, Izabela

AU - Cabral, Ethan

AU - Glogowska, Magdelena

AU - Amato, Carol

AU - Robinson, Steven

AU - Hintzsche, Jennifer

AU - Applegate, Allison

AU - Seelenfreund, Eric

AU - Gonzalez, Rita

AU - Wells, Keith

AU - Bagby, Stacey

AU - Tentler, John

AU - Tan, Aik Choon

AU - Wisell, Joshua

AU - Varella-Garcia, Marileila

AU - Robinson, William

PY - 2017/1/1

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AB - Genomic rearrangements resulting in activating kinase fusions have been increasingly described in a number of cancers including malignant melanoma, but their frequency in specific melanoma subtypes has not been reported. We used break-apart fluorescence in situ hybridization (FISH) to identify genomic rearrangements in tissues from 59 patients with various types of malignant melanoma including acral lentiginous, mucosal, superficial spreading, and nodular. We identified four genomic rearrangements involving the genes BRAF, RET, and ROS1. Of these, three were confirmed by Immunohistochemistry (IHC) or sequencing and one was found to be an ARMC10-BRAF fusion that has not been previously reported in melanoma. These fusions occurred in different subtypes of melanoma but all in tumors lacking known driver mutations. Our data suggest gene fusions are more common than previously thought and should be further explored particularly in melanomas lacking known driver mutations.

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Kinase gene fusions in defined subsets of melanoma

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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