Kinetic analysis of RNA interference for lamin A/C in HeLa cells

Sung Ju You; Sang Hoon Lee; Jae Seung Lee

doi:10.1093/abbs/gmq068

Kinetic analysis of RNA interference for lamin A/C in HeLa cells

Sung Ju You, Sang Hoon Lee, Jae Seung Lee

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

Original language	English
Pages (from-to)	623-627
Number of pages	5
Journal	Acta Biochimica et Biophysica Sinica
Volume	42
Issue number	9
DOIs	https://doi.org/10.1093/abbs/gmq068
Publication status	Published - 2010 Sept

Keywords

RNAi
gene silencing
kinetic analysis
lamin A/C
siRNA

ASJC Scopus subject areas

Biophysics
Biochemistry

Access to Document

10.1093/abbs/gmq068

Cite this

@article{cc6f714a5499434c956b9fb71122441f,

title = "Kinetic analysis of RNA interference for lamin A/C in HeLa cells",

abstract = "We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.",

keywords = "RNAi, gene silencing, kinetic analysis, lamin A/C, siRNA",

author = "You, {Sung Ju} and Lee, {Sang Hoon} and Lee, {Jae Seung}",

year = "2010",

month = sep,

doi = "10.1093/abbs/gmq068",

language = "English",

volume = "42",

pages = "623--627",

journal = "Acta Biochimica et Biophysica Sinica",

issn = "1672-9145",

publisher = "Oxford University Press",

number = "9",

}

TY - JOUR

T1 - Kinetic analysis of RNA interference for lamin A/C in HeLa cells

AU - You, Sung Ju

AU - Lee, Sang Hoon

AU - Lee, Jae Seung

PY - 2010/9

Y1 - 2010/9

N2 - We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

AB - We kinetically analyzed RNA interference (RNAi) for lamin A/C in HeLa cells, assuming suppression and recovery phases of gene expression, and dilution of transfected small interfering RNA (siRNA) by cell divisions. We observed the inhibitory effect of RNAi over a period of 6 days using various siRNA concentrations, and the maximum gene silencing efficiency occurred at Day 2 or 3. The gene silencing efficiency as a function of time and siRNA concentration was further quantitatively evaluated using a kinetic analysis method, demonstrating that RNAi for lamin A/C can be understood as a conventional drug-response system. This work provides potentially important guidelines for future applications using nanomaterials as delivery vehicles of siRNA in RNAi for lamin A/C.

KW - RNAi

KW - gene silencing

KW - kinetic analysis

KW - lamin A/C

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=77956271412&partnerID=8YFLogxK

U2 - 10.1093/abbs/gmq068

DO - 10.1093/abbs/gmq068

M3 - Article

C2 - 20732854

AN - SCOPUS:77956271412

SN - 1672-9145

VL - 42

SP - 623

EP - 627

JO - Acta Biochimica et Biophysica Sinica

JF - Acta Biochimica et Biophysica Sinica

IS - 9

ER -

Kinetic analysis of RNA interference for lamin A/C in HeLa cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this