Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome

Sang Hyun Ra; Min Jae Kim; Min Chul Kim; Se Yoon Park; Seong Yeon Park; Yong Pil Chong; Sang Oh Lee; Sang Ho Choi; Yang Soo Kim; Keun Hwa Lee; Sung Han Kim; Sun Ho Kee

doi:10.3390/v13010006

Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome

Sang Hyun Ra
, Min Jae Kim
, Min Chul Kim
, Se Yoon Park
, Seong Yeon Park
, Yong Pil Chong
, Sang Oh Lee
, Sang Ho Choi
, Yang Soo Kim
, Keun Hwa Lee^*
, Sung Han Kim^*
, Sun Ho Kee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.

Original language	English
Article number	6
Journal	Viruses
Volume	13
Issue number	1
DOIs	https://doi.org/10.3390/v13010006
Publication status	Published - 2021 Jan

Bibliographical note

Funding Information:
This study was supported by grants from the Government-wide R&D Fund Project for Infectious Disease Research (GFID), Korea (grant no. HG18C0037) and Korea National Institute of Health (KNIH), Korea (grant no. 2021-ER5304-01).We thank the participants of this study. We are grateful to Joon Seo Lim for assistance with English editing.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).

Keywords

Enzyme-linked immunosorbent assay (ELISA)
Immunofluorescence assay (IFA)
Immunoglobulin G (IgG)
Immunoglobulin M (IgM)
Severe fever with thrombocytopenia syndrome (SFTS)
Viral load

ASJC Scopus subject areas

Infectious Diseases
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v13010006

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@article{7b57b56717d241bab160c19e0d45b79d,

title = "Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome",

abstract = "Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63\%, 76\%, 90\%, and 100\%, and 58\%, 86\%, 100\%, and 100\%, respectively, for IgG, whereas they were 32\%, 62\%, 80\%, and 100\%, and 53\%, 62\%, 70\%, and 100\%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.",

keywords = "Enzyme-linked immunosorbent assay (ELISA), Immunofluorescence assay (IFA), Immunoglobulin G (IgG), Immunoglobulin M (IgM), Severe fever with thrombocytopenia syndrome (SFTS), Viral load",

author = "Ra, \{Sang Hyun\} and Kim, \{Min Jae\} and Kim, \{Min Chul\} and Park, \{Se Yoon\} and Park, \{Seong Yeon\} and Chong, \{Yong Pil\} and Lee, \{Sang Oh\} and Choi, \{Sang Ho\} and Kim, \{Yang Soo\} and Lee, \{Keun Hwa\} and Kim, \{Sung Han\} and Kee, \{Sun Ho\}",

note = "Funding Information: This study was supported by grants from the Government-wide R\&D Fund Project for Infectious Disease Research (GFID), Korea (grant no. HG18C0037) and Korea National Institute of Health (KNIH), Korea (grant no. 2021-ER5304-01).We thank the participants of this study. We are grateful to Joon Seo Lim for assistance with English editing. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).",

year = "2021",

month = jan,

doi = "10.3390/v13010006",

language = "English",

volume = "13",

journal = "Viruses",

issn = "1999-4915",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "1",

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T1 - Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome

AU - Ra, Sang Hyun

AU - Kim, Min Jae

AU - Kim, Min Chul

AU - Park, Se Yoon

AU - Park, Seong Yeon

AU - Chong, Yong Pil

AU - Lee, Sang Oh

AU - Choi, Sang Ho

AU - Kim, Yang Soo

AU - Lee, Keun Hwa

AU - Kim, Sung Han

AU - Kee, Sun Ho

N1 - Funding Information: This study was supported by grants from the Government-wide R&D Fund Project for Infectious Disease Research (GFID), Korea (grant no. HG18C0037) and Korea National Institute of Health (KNIH), Korea (grant no. 2021-ER5304-01).We thank the participants of this study. We are grateful to Joon Seo Lim for assistance with English editing. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/).

PY - 2021/1

Y1 - 2021/1

N2 - Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.

AB - Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.

KW - Enzyme-linked immunosorbent assay (ELISA)

KW - Immunofluorescence assay (IFA)

KW - Immunoglobulin G (IgG)

KW - Immunoglobulin M (IgM)

KW - Severe fever with thrombocytopenia syndrome (SFTS)

KW - Viral load

UR - https://www.scopus.com/pages/publications/85099115884

U2 - 10.3390/v13010006

DO - 10.3390/v13010006

M3 - Article

C2 - 33375753

AN - SCOPUS:85099115884

SN - 1999-4915

VL - 13

JO - Viruses

JF - Viruses

IS - 1

M1 - 6

ER -

Kinetics of serological response in patients with severe fever with thrombocytopenia syndrome

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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