Knockdown of apoptosis signal-regulating kinase 1 modulates basal glycogen synthase kinase-3β kinase activity and regulates cell migration

Kyung Tae Noh; Ssang Goo Cho; Eui Ju Choi

doi:10.1016/j.febslet.2010.08.029

Knockdown of apoptosis signal-regulating kinase 1 modulates basal glycogen synthase kinase-3β kinase activity and regulates cell migration

Kyung Tae Noh, Ssang Goo Cho, Eui Ju Choi

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

GSK-3β is a basally active kinase. Axin forms a complex with GSK-3β and β-catenin; this complex promotes the GSK-3β-dependent phosphorylation of β-catenin, thereby inducing its degradation. However, the inhibition of GSK-3β provokes cell migration via the dysregulation of β-catenin. In this study, we determined that the level of apoptosis signal-regulating kinase 1 (ASK1) was lower in a metastatic breast cancer cell line, compared to that of non-metastatic cancer cell lines and the knockdown of ASK1 not only induces β-catenin activation via the inhibition of GSK-3β and collapsing the subsequent protein complex by regulating Axin dynamics, but also stimulates cell migration. Together, the blockage of the GSK-3β-β-catenin pathway resulting from the knockdown of ASK1 modulates the migration of breast cancer cells.

Original language	English
Pages (from-to)	4097-4101
Number of pages	5
Journal	FEBS Letters
Volume	584
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2010.08.029
Publication status	Published - 2010 Sept

Bibliographical note

Funding Information:
We would like to thank Dr. Woo Keun Song (GIST of South Korea) for the pTopflash plasmid. This work was supported by a NRF Grant (2010-0001197), by the Original Technology Research Program for Brain Science (2010-0002153) and the Mid-career Researcher Program (2010-0000488) through NRF Grant funded by the Ministry of Education, Science & Technology (MEST) of the Korea (E.-J.C.).

Keywords

ASK1
Axin
GSK-3β
Migration
β-Catenin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2010.08.029

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title = "Knockdown of apoptosis signal-regulating kinase 1 modulates basal glycogen synthase kinase-3β kinase activity and regulates cell migration",

abstract = "GSK-3β is a basally active kinase. Axin forms a complex with GSK-3β and β-catenin; this complex promotes the GSK-3β-dependent phosphorylation of β-catenin, thereby inducing its degradation. However, the inhibition of GSK-3β provokes cell migration via the dysregulation of β-catenin. In this study, we determined that the level of apoptosis signal-regulating kinase 1 (ASK1) was lower in a metastatic breast cancer cell line, compared to that of non-metastatic cancer cell lines and the knockdown of ASK1 not only induces β-catenin activation via the inhibition of GSK-3β and collapsing the subsequent protein complex by regulating Axin dynamics, but also stimulates cell migration. Together, the blockage of the GSK-3β-β-catenin pathway resulting from the knockdown of ASK1 modulates the migration of breast cancer cells.",

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author = "Noh, {Kyung Tae} and Cho, {Ssang Goo} and Choi, {Eui Ju}",

note = "Funding Information: We would like to thank Dr. Woo Keun Song (GIST of South Korea) for the pTopflash plasmid. This work was supported by a NRF Grant (2010-0001197), by the Original Technology Research Program for Brain Science (2010-0002153) and the Mid-career Researcher Program (2010-0000488) through NRF Grant funded by the Ministry of Education, Science & Technology (MEST) of the Korea (E.-J.C.). ",

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doi = "10.1016/j.febslet.2010.08.029",

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AU - Choi, Eui Ju

N1 - Funding Information: We would like to thank Dr. Woo Keun Song (GIST of South Korea) for the pTopflash plasmid. This work was supported by a NRF Grant (2010-0001197), by the Original Technology Research Program for Brain Science (2010-0002153) and the Mid-career Researcher Program (2010-0000488) through NRF Grant funded by the Ministry of Education, Science & Technology (MEST) of the Korea (E.-J.C.).

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N2 - GSK-3β is a basally active kinase. Axin forms a complex with GSK-3β and β-catenin; this complex promotes the GSK-3β-dependent phosphorylation of β-catenin, thereby inducing its degradation. However, the inhibition of GSK-3β provokes cell migration via the dysregulation of β-catenin. In this study, we determined that the level of apoptosis signal-regulating kinase 1 (ASK1) was lower in a metastatic breast cancer cell line, compared to that of non-metastatic cancer cell lines and the knockdown of ASK1 not only induces β-catenin activation via the inhibition of GSK-3β and collapsing the subsequent protein complex by regulating Axin dynamics, but also stimulates cell migration. Together, the blockage of the GSK-3β-β-catenin pathway resulting from the knockdown of ASK1 modulates the migration of breast cancer cells.

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Knockdown of apoptosis signal-regulating kinase 1 modulates basal glycogen synthase kinase-3β kinase activity and regulates cell migration

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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