L-Fucose Synthesis Using a Halo- and Thermophilic L-Fucose Isomerase from Polyextremophilic Halothermothrix orenii

In Jung Kim, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


L-Fucose isomerase (L-FucI)-mediated isomerization is a promising biotechnological approach for synthesizing various rare sugars of industrial significance, including L-fucose. Extremozymes that can retain their functional conformation under extreme conditions, such as high temperature and salinity, offer favorable applications in bioprocesses that accompany harsh conditions. To date, only one thermophilic L-FucI has been characterized for L-fucose synthesis. Here, we report L-FucI from Halothermothrix orenii (HoFucI) which exhibits both halophilic and thermophilic properties. When evaluated under various biochemical conditions, HoFucI exhibited optimal activities at 50–60 °C and pH 7 with 0.5–1 M NaCl in the presence of 1 mM Mn2+ as a cofactor. The results obtained here show a unique feature of HoFucI as a polyextremozyme, which facilitates the biotechnological production of L-fucose using this enzyme.

Original languageEnglish
Article number4029
JournalApplied Sciences (Switzerland)
Issue number8
Publication statusPublished - 2022 Apr 1

Bibliographical note

Funding Information:
Funding: This work was supported by the Mid‐career Researcher Program through the National Research Foundation of Korea (NRF) (2020R1A2B5B02002631) and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries, funded by the Ministry of Agriculture, Food, and Rural Affairs (32136‐05‐1‐SB010). I.J.K. acknowledges the grant from the NRF (NRF‐2020R1A6A3A03039153). This work was also supported by the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety and by a Korea University Grant.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.


  • Halothermothrix orenii
  • L-fucose
  • L-fucose isomerase
  • L-fuculose
  • extremophile
  • halothermophilic bacteria

ASJC Scopus subject areas

  • General Materials Science
  • Instrumentation
  • General Engineering
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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