Label-free and live cell imaging by interferometric scattering microscopy

Jin Sung Park, Il Buem Lee, Hyeon Min Moon, Jong Hyeon Joo, Kyoung Hoon Kim, Seok Cheol Hong, Minhaeng Cho

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena.

Original languageEnglish
Pages (from-to)2690-2697
Number of pages8
JournalChemical Science
Issue number10
Publication statusPublished - 2018

Bibliographical note

Funding Information:
This work was supported by IBS-R023-D1 and NRF-2016R1A2B4 014855 (S.-C. H.).

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • General Chemistry


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