Label-free imaging of membrane potential using membrane electromotility

Seungeun Oh, Christopher Fang-Yen, Wonshik Choi, Zahid Yaqoob, Dan Fu, Yongkeun Park, Ramachandra R. Dassari, Michael S. Feld

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.

Original languageEnglish
Pages (from-to)11-18
Number of pages8
JournalBiophysical Journal
Issue number1
Publication statusPublished - 2012 Jul 3

Bibliographical note

Funding Information:
This work was funded by the National Center for Research Resources of the National Institutes of Health (P41-RR02594-24), the National Science Foundation (DBI-0754339), and Hamamatsu Corporation.

ASJC Scopus subject areas

  • Biophysics


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