TY - GEN
T1 - Label-free, microfluidic separation of human breast carcinoma and epithelial cells by adhesion difference
AU - Kwon, Keon Woo
AU - Lee, Sang Ho
AU - Kim, Byungkyu
AU - Park, Min Cheol
AU - Kim, Pilnam
AU - Suh, Kahp Y.
PY - 2007
Y1 - 2007
N2 - A simple, label-free microfluidic separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and parallel lines with respect to the direction of flow) were fabricated using UV-assisted capillary moulding onto glass substrate of PDMS microfluidic channel. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. The adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of 300 μl/min
AB - A simple, label-free microfluidic separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50nm pillars, 50nm perpendicular and parallel lines with respect to the direction of flow) were fabricated using UV-assisted capillary moulding onto glass substrate of PDMS microfluidic channel. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. The adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50nm parallel line pattern followed by flow-induced detachment at a flow rate of 300 μl/min
KW - Cell adhesion
KW - Cell separation
KW - Microfluidics
KW - Nanostructures
UR - http://www.scopus.com/inward/record.url?scp=50049130725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049130725&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300226
DO - 10.1109/SENSOR.2007.4300226
M3 - Conference contribution
AN - SCOPUS:50049130725
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 699
EP - 702
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Y2 - 10 June 2007 through 14 June 2007
ER -
