Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm

Moonseok Kim, Yonghyeon Jo, Jin Hee Hong, Suhyun Kim, Seokchan Yoon, Kyung Deok Song, Sungsam Kang, Byunghak Lee, Guang Hoon Kim, Hae Chul Park, Wonshik Choi

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical.

Original languageEnglish
Article number3152
JournalNature communications
Issue number1
Publication statusPublished - 2019 Dec 1

Bibliographical note

Funding Information:
This research was supported by IBS-R023-D1, the National Research Foundation of Korea (2019R1C1C1008175, 2016R1A6A3A11936389, 2016R1D1A1B03933770), the Catholic Medical Center Research Foundation made in the program year of 2018, and the KERI Primary research program of MSIT/NST (No. 18-12-N0101-41). We are grateful to Myunghwan Choi for constructive discussion.

Publisher Copyright:
© 2019, The Author(s).

ASJC Scopus subject areas

  • General
  • General Physics and Astronomy
  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology


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