This work investigates the intrinsic cell labeling efficiency of the Fe3O4 nanoparticles prepared by a modified thermal decomposition method using nontoxic precursors and a biocompatible polymer surfactant. This method eliminates the current need for additional step of surface modification. The structural analysis reveals the highly crystalline feature of the nanoparticles, while the magnetic measurement shows their superparamagnetic behavior at room temperature. Fe3O4 nanoparticles were efficiently incorporated into the murine macrophage cells (RAW264.7) without visible cytotoxicity. Cell labeling efficiency was found to be over 90 as measured by magnetically activated cell sorting and physical property measurement system. Therefore, such Fe3O4 nanoparticles could provide a useful magnetic cell labeling tool for macrophage cells using their phagocytic/endocytic activity and further apply to the other relevant biomedical applications.
Bibliographical noteFunding Information:
This work was supported by the Pioneer Research Center Program (2010-0002191), the Converging Research Center Program (2010K001437) and the grant (2010-0017950) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology, the Industrial Core Technology Development Program funded by the Ministry of Knowledge Economy (No. 10033183), the Seoul R&BD Program (No. 10920), the Innovative Research Institute for Cell Therapy (A062260) and a grant from KICOS (K20704000007-10A0500-00710).
ASJC Scopus subject areas
- General Physics and Astronomy