Introduction: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) levels were previously reported, and further, these conflicting results were suggested to be partly accounted for smoking status of subjects. However, the genetic contribution of Glu298Asp (G894T) polymorphism to plasma NO levels with respect to smoking status has not been published. Methods: In a group of 411 healthy Korean subjects aged 19-81 years, the end product of NO (NOx: nitrite plus nitrate) as an index of plasma NO levels was measured by the Griess method. The genotypes of G894T polymorphism were determined by the banding patterns on gel electrophoresis after restriction enzyme digestion. Results: Comparison of plasma NOx levels revealed no significant differences across the genotypes and alleles of G894T polymorphism, which is independently of smoking status. However, significant differences in plasma NOx levels between nonsmokers and smokers were observed (P=0.0040). Furthermore, only the common G allele was found to be responsible for these differences. Multiple regression analysis showed that the most independent contributing factor for plasma NOx levels was smoking (P=0.0119) and followed by triglycerides (P=0.0384). Conclusions: Our results indicate no substantial effect of G894T polymorphism on the variance of plasma NOx levels in healthy Korean population.
Bibliographical noteFunding Information:
This work was supported in part by the Korean National Institute of Health intramural research grant (334-6113-211-207-00) and Science Research Center grant from the Korean Science and Engineering Foundation (KOSEF) to the Nitric Oxide Radical Toxicology Research Center (NORTReC) to Dr. Inho Jo, by Korea University Institutes of Medical Science grant (2000-n6) to Dr. Chol Shin, and by the Biomedical Brain Research Center grant from the Ministry of Health and Welfare to Dr. Sangmee Ahn Jo. We thank Dr. Younjhin Ahn and Mr. Jungbok Lee for their valuable discussions regarding statistical analysis, and Ms. Jooyoung Lee for secretarial assistance.
- Endothelial nitric oxide synthase gene
- Plasma nitric oxide level
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