@article{4cbcf4fec3344954bbf95396e1b946ff,
title = "Large and Externally Positioned Ligand-Coated Nanopatches Facilitate the Adhesion-Dependent Regenerative Polarization of Host Macrophages",
abstract = "Macrophages can associate with extracellular matrix (ECM) demonstrating nanosequenced cell-adhesive RGD ligand. In this study, we devised barcoded materials composed of RGD-coated gold and RGD-absent iron nanopatches to show various frequencies and position of RGD-coated nanopatches with similar areas of iron and RGD-gold nanopatches that maintain macroscale and nanoscale RGD density invariant. Iron patches were used for substrate coupling. Both large (low frequency) and externally positioned RGD-coated nanopatches stimulated robust attachment in macrophages, compared with small (high frequency) and internally positioned RGD-coated nanopatches, respectively, which mediate their regenerative/anti-inflammatory M2 polarization. The nanobarcodes exhibited stability in vivo. We shed light into designing ligand-engineered nanostructures in an external position to facilitate host cell attachment, thereby eliciting regenerative host responses.",
keywords = "RGD frequency, RGD nanopatches, macrophage adhesion, macrophage polarization",
author = "Sunhong Min and Jeon, {Yoo Sang} and Hyojun Choi and Chandra Khatua and Na Li and Gunhyu Bae and Jung, {Hee Joon} and Yuri Kim and Hyunsik Hong and Jeongeun Shin and Ko, {Min Jun} and Ko, {Han Seok} and Taesoon Kim and Moon, {Jun Hwan} and Jae-Jun Song and Dravid, {Vinayak P.} and Kim, {Young Keun} and Heemin Kang",
note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (Nos. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF-STEM imaging was performed with the support of Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center the International Institute for Nanotechnology (IIN), the Keck Foundation and the State of Illinois, through the IIN. Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (Nos. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF-STEM imaging was performed with the support of Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = oct,
day = "14",
doi = "10.1021/acs.nanolett.0c02655",
language = "English",
volume = "20",
pages = "7272--7280",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "10",
}